Abstract
Heterologous expression of plant metabolic enzymes in microorganisms is extensively used for identifying genes involved in their pathways and producing useful compounds. Here, we describe a plasmid-based yeast expression system that easily allows the expression of different triterpene biosynthetic genes in wild-type yeast, providing a useful platform for identifying their functions and facilitating combinatorial biosynthesis of triterpenoids.
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Acknowledgements
This work was financially aid in part by Frontier Research Base for Global Young Researchers, Osaka University, supported by the Ministry of Education, Culture, Sports, Science, and Technology (MEXT).
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Fukushima, E.O., Seki, H., Muranaka, T. (2014). Heterologous Expression of Triterpene Biosynthetic Genes in Yeast and Subsequent Metabolite Identification Through GC-MS. In: RodrÃguez-Concepción, M. (eds) Plant Isoprenoids. Methods in Molecular Biology, vol 1153. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0606-2_17
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DOI: https://doi.org/10.1007/978-1-4939-0606-2_17
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-0605-5
Online ISBN: 978-1-4939-0606-2
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