Abstract
Terpene specialized metabolites exhibit multiple functions in plant–environment interactions and plant development. Molecular biologists investigating the biochemistry and molecular function of terpenes need to apply robust but yet sensitive analytical methods optimized and adapted to the structural diversity and often varying concentrations of terpene compounds in plant tissues. Here we present hands-on protocols for sample preparation and GC-MS or LC-MS/MS analysis of selected diterpene and triterpene hydrocarbons or oxygenated derivatives from roots and shoots of Arabidopsis and rice.
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Acknowledgement
This work was supported by a National Science Foundation grant MCB-0950865.
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Wang, Q., Sohrabi, R., Tholl, D. (2014). Analysis of Diterpenes and Triterpenes from Plant Foliage and Roots. In: RodrĂguez-ConcepciĂłn, M. (eds) Plant Isoprenoids. Methods in Molecular Biology, vol 1153. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0606-2_10
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DOI: https://doi.org/10.1007/978-1-4939-0606-2_10
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