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Pseudomonas Methods and Protocols pp 245–254Cite as

Biosensors for Qualitative and Semiquantitative Analysis of Quorum Sensing Signal Molecules

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1149))

Abstract

Biosensors are biological tools that can be used to assay bacterial cultures for quorum sensing signal molecules (QSSMs) both qualitatively and semiquantitatively. QSSMs can be extracted from Pseudomonas aeruginosa cultures using organic solvents and tentatively identified via thin layer chromatography in combination with biosensor overlays. Alternatively, QSSMs can be quantified in spent culture supernatants or solvent extracts using biosensor-based spectrophotometric, luminescence, or fluorescence assays.

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Author information

Authors and Affiliations

  1. School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, NG7 2RD, UK

    Matthew Fletcher, Miguel Cámara & Paul Williams

  2. Centre for Analytical Bioscience, School of Pharmacy, University of Nottingham, University Park, Nottingham, NG7 2RD, UK

    David A. Barrett

Authors
  1. Matthew Fletcher
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  2. Miguel Cámara
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  3. David A. Barrett
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  4. Paul Williams
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Corresponding author

Correspondence to Paul Williams .

Editor information

Editors and Affiliations

  1. MRC Centre for Molecular Bacteriology and Infection (CMBI) Department of Life Sciences, Imperial College, London, UK

    Alain Filloux

  2. Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada, Spain

    Juan-Luis Ramos

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Fletcher, M., Cámara, M., Barrett, D.A., Williams, P. (2014). Biosensors for Qualitative and Semiquantitative Analysis of Quorum Sensing Signal Molecules. In: Filloux, A., Ramos, JL. (eds) Pseudomonas Methods and Protocols. Methods in Molecular Biology, vol 1149. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-0473-0_20

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  • DOI: https://doi.org/10.1007/978-1-4939-0473-0_20

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-0472-3

  • Online ISBN: 978-1-4939-0473-0

  • eBook Packages: Springer Protocols

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