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Membrane Proteomics of Pseudomonas aeruginosa

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Pseudomonas Methods and Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1149))

Abstract

In recent years gel-free proteomics approaches have been increasingly used for global quantitative proteome analyses of multiple prokaryotic organisms, including Pseudomonas aeruginosa. A major advantage of this method is its suitability for the investigation of membrane proteomes. In this chapter, we present a protocol for preparation of proteins from the inner and outer membrane of P. aeruginosa PAO1 grown as a biofilm culture. Parameters for quantitative protein measurements by 2D-LC-MS/MS are described.

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Acknowledgement

We thank Uwe Kärst for critically reading the manuscript.

Author information

Authors and Affiliations

  1. Microbial Proteomics, Helmholtz Centre for Infection Research (HZI), Inhoffenstr, Braunschweig, Germany

    Zofia Magnowska, Isabel Hartmann & Lothar Jänsch

  2. Institute of Microbiology, Technische Universität Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany

    Dieter Jahn

Authors
  1. Zofia Magnowska
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  2. Isabel Hartmann
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  3. Lothar Jänsch
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  4. Dieter Jahn
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Corresponding author

Correspondence to Dieter Jahn .

Editor information

Editors and Affiliations

  1. MRC Centre for Molecular Bacteriology and Infection (CMBI) Department of Life Sciences, Imperial College, London, UK

    Alain Filloux

  2. Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada, Spain

    Juan-Luis Ramos

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Magnowska, Z., Hartmann, I., Jänsch, L., Jahn, D. (2014). Membrane Proteomics of Pseudomonas aeruginosa . In: Filloux, A., Ramos, JL. (eds) Pseudomonas Methods and Protocols. Methods in Molecular Biology, vol 1149. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-0473-0_18

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  • DOI: https://doi.org/10.1007/978-1-4939-0473-0_18

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-0472-3

  • Online ISBN: 978-1-4939-0473-0

  • eBook Packages: Springer Protocols

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