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Herpes Simplex Virus pp 181–190Cite as

Characterization of Extracellular HSV-1 Virions by Proteomics

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1144))

Abstract

The analysis of herpes simplex virus type 1 mature extracellular virions by proteomics requires highly enriched samples to limit false positives and favor the detection of true components. The protocol described below involves the removal of highly contaminating serum proteins and purification of the virions by a series of differential and density centrifugation steps. In addition, L-particles, which are viral particles devoid of genome and capsid but present in the extracellular milieu, are depleted on Ficoll 400 gradients. As previously reported, the resulting viral particles are free of most contaminants and suitable for mass spectrometry.

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Acknowledgement

This work was supported by grants from the Canadian Institutes of Health Research (MOP 82921 and MOP 258030). Special thanks to Kerstin Radtke and Nabil El Bilali for proofreading and Christina Bell for the mass spectrometry protocols.

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Authors and Affiliations

  1. Department of Pathology and Cell Biology, University of Montreal, V-541 Pavillon Roger Gaudry, 2900 boul. Édouard-Montpetit, Montreal, QC, Canada, H3C 3J7

    Roger Lippé

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  1. Roger Lippé
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Correspondence to Roger Lippé .

Editor information

Editors and Affiliations

  1. Centre for Virus Research, Westmead Millennium Institute, Westmead, New South Wales, Australia

    Russell J. Diefenbach

  2. Institute of Virology, University of Zürich, Zürich, Switzerland

    Cornel Fraefel

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Lippé, R. (2014). Characterization of Extracellular HSV-1 Virions by Proteomics. In: Diefenbach, R., Fraefel, C. (eds) Herpes Simplex Virus. Methods in Molecular Biology, vol 1144. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0428-0_12

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  • DOI: https://doi.org/10.1007/978-1-4939-0428-0_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0427-3

  • Online ISBN: 978-1-4939-0428-0

  • eBook Packages: Springer Protocols

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