Abstract
DNA delivery to for example skin and muscle can easily be performed with electroporation. The method is efficient, feasible, and inexpensive and the future possibilities are numerous. Here we present our protocol for gene transfection to mouse skin using naked plasmid DNA and electric pulses.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Mir LM, Moller PH, Andre F et al (2005) Electric pulse-mediated gene delivery to various animal tissues. Adv Genet 54:83–114
Hojman P, Gissel H, Gehl J (2007) Sensitive and precise regulation of haemoglobin after gene transfer of erythropoietin to muscle tissue using electroporation. Gene Ther 14:950–959
Gothelf A, Hojman P, Gehl J (2010) Therapeutic levels of erythropoietin (EPO) achieved after gene electrotransfer to skin in mice. Gene Ther 17:1077–1084
Daud AI, DeConti RC, Andrews S et al (2008) Phase I trial of interleukin-12 plasmid electroporation in patients with metastatic melanoma. J Clin Oncol 26:5896–5903
Gothelf A, Gehl J (2012) What you always needed to know about electroporation based DNA vaccines. Hum Vaccin Immunother 8:1694–1702
Cemazar M, Sersa G (2007) Electrotransfer of therapeutic molecules into tissues. Curr Opin Mol Ther 9:554–562
Andre FM, Gehl J, Sersa G et al (2008) Efficiency of high- and low-voltage pulse combinations for gene electrotransfer in muscle, liver, tumor, and skin. Hum Gene Ther 19:1261–1271
Gothelf A, Gehl J (2010) Gene electrotransfer to skin; review of existing literature and clinical perspectives. Curr Gene Ther 10:287–299
Gothelf A, Eriksen J, Hojman P et al (2010) Duration and level of transgene expression after gene electrotransfer to skin in mice. Gene Ther 17:839–845
Gothelf A, Mahmood F, Dagnaes-Hansen F et al (2011) Efficacy of transgene expression in porcine skin as a function of electrode choice. Bioelectrochemistry 82:95–102
Pavselj N, Preat V (2005) DNA electrotransfer into the skin using a combination of one high- and one low-voltage pulse. J Control Release 106:407–415
Rocchetta HL, Boylan CJ, Foley JW et al (2001) Validation of a noninvasive, real-time imaging technology using bioluminescent Escherichia coli in the neutropenic mouse thigh model of infection. Antimicrob Agents Chemother 45:129–137
Colin M, Moritz S, Schneider H et al (2000) Haemoglobin interferes with the ex vivo luciferase luminescence assay: consequence for detection of luciferase reporter gene expression in vivo. Gene Ther 7:1333–1336
Gothelf A, Hojman P, Gehl J (2009) Change in hemoglobin levels due to anesthesia in mice: an important confounder in studies on hematopoietic drugs. Biol Proced Online 11:325–330
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media New York
About this protocol
Cite this protocol
Gothelf, A., Gehl, J. (2014). Electroporation-Based DNA Delivery Technology: Methods for Gene Electrotransfer to Skin. In: Rinaldi, M., Fioretti, D., Iurescia, S. (eds) DNA Vaccines. Methods in Molecular Biology, vol 1143. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0410-5_7
Download citation
DOI: https://doi.org/10.1007/978-1-4939-0410-5_7
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-0409-9
Online ISBN: 978-1-4939-0410-5
eBook Packages: Springer Protocols