Skip to main content
SpringerLink
Log in

DNA Vaccination as a Treatment for Chronic Kidney Disease

  • Protocol
  • First Online:
DNA Vaccines

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1143))

Abstract

Chronic kidney disease is one of the major health problems worldwide. DNA vaccination delivers plasmid DNA encoding the target gene to induce both humoral and cellular immune responses. Here, we describe the methods of CD40 DNA vaccine enhanced by dendritic cell (DC) targeting on the development of Heymann nephritis (HN), a rat model of human membranous nephropathy.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Wu H, Walters G, Knight JF et al (2003) DNA vaccination against specific pathogenic TCRs reduces proteinuria in active Heymann nephritis by inducing specific autoantibodies. J Immunol 171:4824–4829

    Article  CAS  PubMed  Google Scholar 

  2. Wu H, Wang Y, Tay YC et al (2005) DNA vaccination with naked DNA encoding MCP-1 and RANTES protects against renal injury in adriamycin nephropathy. Kidney Int 67:2178–2186

    Article  CAS  PubMed  Google Scholar 

  3. Zheng G, Wang Y, Xiang SH et al (2006) DNA vaccination with CCL2 DNA modified by the addition of an adjuvant epitope protects against “nonimmune” toxic renal injury. J Am Soc Nephrol 17:465–474

    Article  CAS  PubMed  Google Scholar 

  4. Nchinda G, Kuroiwa J, Oks M et al (2008) The efficacy of DNA vaccination is enhanced in mice by targeting the encoded protein to dendritic cells. J Clin Invest 118:1427–1436

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  5. Wang Y, Wang YM, Zheng G et al (2013) DNA vaccine encoding CD40 targeted to dendritic cells in situ prevents the development of Heymann nephritis in rats. Kidney Int 83:223–232

    Article  CAS  PubMed  Google Scholar 

  6. Wang YM, Zhou JJ, Wang Y et al (2013) Daedalic DNA vaccination against self antigens as a treatment for chronic kidney disease. Int J Clin Exp Pathol 6:326–333

    CAS  PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgement

This work was supported by research grants from the National Health and Medical Research Council of Australia (NHMRC grant 571343 and 632517). We thank the animal house staff in the Westmead Hospital Animal House Facility for care of the animals. We thank Godwin Nchinda (The Rockefeller University) for kindly providing the DEC205 vectors. We thank Virginia James (Westmead Millennium Institute, Australia) for her technical assistances in histology.

Author information

Authors and Affiliations

  1. Centre for Kidney Research, Children’s Hospital at Westmead, The University of Sydney, Level 2, Crn Hawkesbury Road, Hainsworth Street, Sydney, NSW, 2145, Australia

    Yuan Min Wang Ph.D. & Stephen I. Alexander

Authors
  1. Yuan Min Wang Ph.D.
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Stephen I. Alexander
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yuan Min Wang Ph.D. .

Editor information

Editors and Affiliations

  1. Section Of Medical Biotechnology, Institute of Translational Pharmacology (IFT), National Research Council (CNR), Rome, Italy

    Monica Rinaldi

  2. Section Of Medical Biotechnology, Institute of Translational Pharmacology (IFT), National Research Council (CNR), Rome, Italy

    Daniela Fioretti

  3. Section Of Medical Biotechnology, Institute of Translational Pharmacology (IFT), National Research Council (CNR), Rome, Italy

    Sandra Iurescia

Rights and permissions

Reprints and permissions

Copyright information

© 2014 Springer Science+Business Media New York

About this protocol

Cite this protocol

Wang, Y.M., Alexander, S.I. (2014). DNA Vaccination as a Treatment for Chronic Kidney Disease. In: Rinaldi, M., Fioretti, D., Iurescia, S. (eds) DNA Vaccines. Methods in Molecular Biology, vol 1143. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0410-5_18

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-0410-5_18

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0409-9

  • Online ISBN: 978-1-4939-0410-5

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation