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Microneedle Applications for DNA Vaccine Delivery to the Skin

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DNA Vaccines

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1143))

Abstract

Microneedles were initially developed as pretreatment tools for the delivery of therapeutic drugs to intradermal locales in the human skin. Over time, variations in microneedle forms and functions burgeoned through the works of many researchers worldwide. The four major types of microneedles in use today are solid, dissolving, coating, and hollow microneedles. The emergence of different types of microneedles also paved the way for a flourishing diversification of microneedle applications, one of the most remarkable of which deals with the transcutaneous delivery of prophylactic vaccines. Here, we describe fabrication methods of microneedles and DNA vaccine loading methods on the microneedle surface. Furthermore, in the latter part of this chapter, in vivo test protocols for assessing the efficacy of gene delivery using microneedles are described.

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 373-1 Guseong-dong, Yuseong-gu, Daejeon, South Korea

    Hae-yong Seok, Hyemee Suh, Sunghyun Baek & Yeu-Chun Kim

Authors
  1. Hae-yong Seok
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  2. Hyemee Suh
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  3. Sunghyun Baek
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  4. Yeu-Chun Kim
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Corresponding author

Correspondence to Yeu-Chun Kim .

Editor information

Editors and Affiliations

  1. Section Of Medical Biotechnology, Institute of Translational Pharmacology (IFT), National Research Council (CNR), Rome, Italy

    Monica Rinaldi

  2. Section Of Medical Biotechnology, Institute of Translational Pharmacology (IFT), National Research Council (CNR), Rome, Italy

    Daniela Fioretti

  3. Section Of Medical Biotechnology, Institute of Translational Pharmacology (IFT), National Research Council (CNR), Rome, Italy

    Sandra Iurescia

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Seok, Hy., Suh, H., Baek, S., Kim, YC. (2014). Microneedle Applications for DNA Vaccine Delivery to the Skin. In: Rinaldi, M., Fioretti, D., Iurescia, S. (eds) DNA Vaccines. Methods in Molecular Biology, vol 1143. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0410-5_10

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  • DOI: https://doi.org/10.1007/978-1-4939-0410-5_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0409-9

  • Online ISBN: 978-1-4939-0410-5

  • eBook Packages: Springer Protocols

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