Leishmania Metacaspase: An Arginine-Specific Peptidase
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The purpose of this chapter is to give insights into metacaspase of Leishmania protozoan parasites as arginine-specific cysteine peptidase. The physiological role of metacaspase in Leishmania is still a matter of debate, whereas its peptidase enzymatic activity has been well characterized. Among the different possible expression systems, metacaspase-deficient yeast cells (Δyca1) have been instrumental in studying the activity of Leishmania major metacaspase (LmjMCA). Here, we describe techniques for purification of LmjMCA and its activity measurement, providing a platform for further identification of LmjMCA substrates.
Key wordsLeishmania Cysteine peptidase Arginine-specific peptidase Metacaspase Enzymatic assay Protease inhibitors
The authors are grateful to the members of the Fasel’s group and to Dr. Frank Madeo who provided the pFM21 construct, which served as a control. This work was funded by the grants FNRS N° 3100A0-116665/1 and IZ70Z0-131421 to NF.
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