Abstract
In contrast to cell-based protein expression, cell-free production is highly consistent, scalable, and amenable to automation. Robots can handle many samples and perform repetitive procedures that are otherwise prone to human error. Here is described commercially available robotics for a wheat germ cell-free system with emphasis on practical applications for structural and functional studies. In addition, described is a cell-free method for preparing protein complexes.
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Acknowledgments
This work was made possible by funding from NIGMS PSI-II grant U54 GM074901 to J.L.M. at the Center for Eukaryotic Structural Genomics (CESG), and PSI-Biology grants U54 GM094584 to the Transmembrane Protein Center (TMPC; B.G.F.) and U01 GM094622 to the Mitochondrial Protein Partnership (MPP; J.L.M.). We thank the many members of CESG, TMPC, and MPP and our biology collaborators for their invaluable contributions.
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Beebe, E.T., Makino, Si., Markley, J.L., Fox, B.G. (2014). Automated Cell-Free Protein Production Methods for Structural Studies. In: Anderson, W.F. (eds) Structural Genomics and Drug Discovery. Methods in Molecular Biology, vol 1140. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-0354-2_9
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DOI: https://doi.org/10.1007/978-1-4939-0354-2_9
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