Abstract
Validation of a mouse model of dengue virus (DENV) infection relies on verification of viremia and productive replication in mouse tissues following infection. Here, we describe a quantitative assay for determining viral RNA levels in mouse serum and tissues. For the purpose of confirming DENV replication, we outline a fluorescence immunohistochemistry (FIHC) protocol for staining a nonstructural protein of DENV.
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Acknowledgements
We would like to thank the contribution of Tyler Prestwood, Malika Morar, and Monica May, past members of our laboratory who contributed to the development of the methods presented here.
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Plummer, E., Shresta, S. (2014). Animal Models in Dengue. In: Padmanabhan, R., Vasudevan, S. (eds) Dengue. Methods in Molecular Biology, vol 1138. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0348-1_23
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DOI: https://doi.org/10.1007/978-1-4939-0348-1_23
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-0348-1
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