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Analysis of Affinity of Dengue Virus Protein Interaction Using Biacore

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Dengue

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1138))

Abstract

Surface plasmon resonance (SPR) biosensors have become the mainstream method for biomolecular interaction analysis. It offers many advantages over conventional methods by its label-free, real-time monitoring, low sample consumption, high throughput, and remarkable sensitivity. We have examined dengue virus protein interactions in the context of antibody affinity measurement, protein–protein interaction, and in the screening of small molecule inhibitors as well as the characterization of the interactions between the small molecule binders and the relevant dengue protein. Here we describe the basic methods involved in performing SPR assays as well as in data processing and evaluation using some examples of dengue proteins.

An erratum to this chapter is available at http://dx.doi.org/10.1007/978-1-4939-0348-1_25

An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-4939-0348-1_25

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Acknowledgements

This work was partly supported by research grants awarded to Dr. Subhash Vasudevan (DUKE-NUS, Singapore) and Dr. Julien Lescar (School of Biological Sciences, Nanyang Technological University, Singapore).

Author information

Authors and Affiliations

  1. Division of Chemical Biology and Biotechnology, School of Biological Sciences, Nanyang Technological University, Singapore, Singapore

    Yin Hoe Yau & Susana Geifman Shochat

Authors
  1. Yin Hoe Yau
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  2. Susana Geifman Shochat
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Editor information

Editors and Affiliations

  1. Georgetown University Medical Center Dept. of Microbiology and Immunology, Washington, District of Columbia, USA

    Radhakrishnan Padmanabhan

  2. Duke-NUS GMS Singapore Emerging Infectious Diseases, Singapore, Singapore

    Subhash G. Vasudevan

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Yau, Y.H., Shochat, S.G. (2014). Analysis of Affinity of Dengue Virus Protein Interaction Using Biacore. In: Padmanabhan, R., Vasudevan, S. (eds) Dengue. Methods in Molecular Biology, vol 1138. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0348-1_17

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  • DOI: https://doi.org/10.1007/978-1-4939-0348-1_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0347-4

  • Online ISBN: 978-1-4939-0348-1

  • eBook Packages: Springer Protocols

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