Abstract
Genetic modification of primary mouse T cells with chimeric antigen receptors (CAR) has emerged as an important tool for optimizing adoptive T cell immunotherapy strategies. However, limitations in current protocols for generating highly pure and sufficient numbers of enriched effector and helper CAR+ T cell subsets remain problematic. Here, we describe a new retroviral transduction protocol for successfully generating transduced CD8+ and CD4+ T lymphocytes for in vitro and in vivo characterization.
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John, L.B., Chee, T.M., Gilham, D.E., Darcy, P.K. (2014). Genetic Modification of Mouse Effector and Helper T Lymphocytes Expressing a Chimeric Antigen Receptor. In: Lawman, M., Lawman, P. (eds) Cancer Vaccines. Methods in Molecular Biology, vol 1139. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0345-0_16
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DOI: https://doi.org/10.1007/978-1-4939-0345-0_16
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-0344-3
Online ISBN: 978-1-4939-0345-0
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