Abstract
Chronic exposure to moderate hypoxia elicits structural and functional changes in the microvascular network of the mammalian CNS. Hypoxia-induced angiogenesis can be elicited and studied by a relatively simple experimental method. Rats or mice can be exposed to mild hypoxia in a hypobaric chamber, or alternatively in a normobaric hypoxia chamber. After 3 weeks, the animals are perfused and fixed, the brain removed, and paraffin embedded and sectioned at 5 μm. The sections are stained for the blood-brain barrier glucose transporter (GLUT-1) by immunohistochemistry, capillary profiles identified and counted as a measure of angiogenesis.
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References
LaManna JC et al (2004) Structural and functional adaptation to hypoxia in the rat brain. J Exp Biol 207(Pt 18):3163–3169
Dore-Duffy P, LaManna JC (2007) Physiologic angiodynamics in the brain. Antioxid Redox Signal 9(9):1363–1371
LaManna JC et al (1992) Brain adaptation to chronic hypobaric hypoxia in rats. J Appl Physiol 72(6):2238–2243
Harik N et al (1996) Time-course and reversibility of the hypoxia-induced alterations in cerebral vascularity and cerebral capillary glucose transporter density. Brain Res 737(1–2):335–338
LaManna JC et al (2004) Structural and functional adaptation to hypoxia in the rat brain. J Exp Biol 207(Pt 18):3163–3169
Millet GP et al (2012) Last word on point: counterpoint: hypobaric hypoxia induces different responses from normobaric hypoxia. J Appl Physiol 112(10):1795
Harik SI et al (1990) Immunocytochemical localization of the erythroid glucose transporter: abundance in tissues with barrier functions. J Neurosci 10:3862–3872
Wright BM (1964) Apparatus for exposing animals to reduced atmospheric pressure for long periods. Br J Haematol 10:75–77
Ward NL et al (2007) Cerebral angiogenic factors, angiogenesis, and physiological response to chronic hypoxia differ among four commonly used mouse strains. J Appl Physiol 102(5):1927–1935
Puchowicz MA et al (2005) Adaptation to chronic hypoxia during diet-induced ketosis. Adv Exp Med Biol 566:51–57
Puchowicz MA et al (2007) Diet-induced ketosis increases capillary density without altered blood flow in rat brain. Am J Physiol Endocrinol Metab 292(6):E1607–E1615
Benderro GF, LaManna JC (2011) Hypoxia-induced angiogenesis is delayed in aging mouse brain. Brain Res 1389:50–60
Paxinos G, Franklin KBJ (2001) The mouse brain in stereotaxic coordinates. Academic, San Diego
Paxinos G, Watson C (1986) The rat brain in stereotaxic coordinates. Academic, San Diego
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Tsipis, C.P., Sun, X., Xu, K., LaManna, J.C. (2014). Hypoxia-Induced Angiogenesis and Capillary Density Determination. In: Milner, R. (eds) Cerebral Angiogenesis. Methods in Molecular Biology, vol 1135. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0320-7_6
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DOI: https://doi.org/10.1007/978-1-4939-0320-7_6
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-0319-1
Online ISBN: 978-1-4939-0320-7
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