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Assays to Examine Endothelial Cell Migration, Tube Formation, and Gene Expression Profiles

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Cerebral Angiogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1135))

Abstract

Common methods for studying angiogenesis in vitro include the tube formation assay, the migration assay, and the study of the endothelial genome. The formation of capillary-like tubes in vitro on basement membrane matrix mimics many steps of the angiogenesis process in vivo and is used widely as a screening test for angiogenic or antiangiogenic factors. Other assays related to the study of angiogenesis include the cell migration assay, the study of gene expression changes during the process of angiogenesis, and the study of endothelial-derived microparticles. Protocols for these procedures will be described here.

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Author information

Authors and Affiliations

  1. Neuroprotection Research Laboratory, Department of Radiology and Neurology, Harvard Medical School, Massachusetts General Hospital, Boston, MA, USA

    Shuzhen Guo, Yi Liu, Kazuhide Hayakawa, Wendy Leung, Changhong Xing & Eng H. Lo

  2. Department of Pediatrics, Harvard Medical School, Massachusetts General Hospital, Boston, MA, USA

    Josephine Lok

  3. Cerebrovascular Research Center, Xuanwu Hospital, Capital Medical University, Beijing, China

    Xunming Ji

Authors
  1. Shuzhen Guo
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  2. Josephine Lok
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  3. Yi Liu
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  4. Kazuhide Hayakawa
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  5. Wendy Leung
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  6. Changhong Xing
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  7. Xunming Ji
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  8. Eng H. Lo
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Editor information

Editors and Affiliations

  1. Scripps Research Institute, La Jolla, California, USA

    Richard Milner

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© 2014 Springer Science+Business Media New York

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Guo, S. et al. (2014). Assays to Examine Endothelial Cell Migration, Tube Formation, and Gene Expression Profiles. In: Milner, R. (eds) Cerebral Angiogenesis. Methods in Molecular Biology, vol 1135. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0320-7_32

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  • DOI: https://doi.org/10.1007/978-1-4939-0320-7_32

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0319-1

  • Online ISBN: 978-1-4939-0320-7

  • eBook Packages: Springer Protocols

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