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Generation of Primary Cultures of Bovine Brain Endothelial Cells and Setup of Cocultures with Rat Astrocytes

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Cerebral Angiogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1135))

Abstract

In vitro models of the blood–brain barrier are useful tools to study blood–brain barrier function as well as drug permeation from the systemic circulation to the brain parenchyma. However, a large number of the available in vitro models fail to reflect the tightness of the in vivo blood–brain barrier. The present protocol describes the setup of an in vitro coculture model based on primary cultures of endothelial cells from bovine brain microvessels and primary cultures of rat astrocytes. The model displays a high electrical tightness and expresses blood–brain barrier marker proteins.

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Authors and Affiliations

  1. Department of Pharmacy, The Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

    Hans C. Helms & Birger Brodin

Authors
  1. Hans C. Helms
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  2. Birger Brodin
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Editor information

Editors and Affiliations

  1. Scripps Research Institute, La Jolla, California, USA

    Richard Milner

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Helms, H.C., Brodin, B. (2014). Generation of Primary Cultures of Bovine Brain Endothelial Cells and Setup of Cocultures with Rat Astrocytes. In: Milner, R. (eds) Cerebral Angiogenesis. Methods in Molecular Biology, vol 1135. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0320-7_30

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  • DOI: https://doi.org/10.1007/978-1-4939-0320-7_30

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0319-1

  • Online ISBN: 978-1-4939-0320-7

  • eBook Packages: Springer Protocols

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