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Plant Epigenetics pp 189–196Cite as

Small RNA Library Preparation and Illumina Sequencing in Plants

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1456))

Abstract

The discovery of small RNAs in plants and animals almost two decades ago attracted a significant interest towards epigenetic regulation of gene expression and the practical implementation of the gained knowledge in applied studies. New and sometimes unexpected functions have been ascribed to sRNAs almost every couple of years since their discovery, hence indicating that the complete role of sRNAs in plant and animal physiology is still barely understood. Next-generation sequencing technologies allow to generate high-resolution profiles of sRNAs for the consequent analysis and possibly to discover novel functions of sRNAs. In this chapter, we provide brief guidelines for sRNA library preparation in plants and a practical approach that can be implemented to overcome possible difficulties with sequencing library generation.

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Acknowledgements

We acknowledge the financial support of Alberta Innovates Biosolutions and Natural Sciences and Engineering Research Council of Canada grants to Igor Kovalchuk and Alberta Innovates Technology Futures for scholarship to Andriy Bilichak. We thank Valentina Titova for proofreading the manuscript.

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Authors and Affiliations

  1. Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, Canada

    Andriy Bilichak

  2. Department of Biological Sciences, University of Lethbridge, 4401 University Drive, Lethbridge, AB, Canada, T1K 3M4

    Andrey Golubov & Igor Kovalchuk

Authors
  1. Andriy Bilichak
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  2. Andrey Golubov
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  3. Igor Kovalchuk
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Corresponding author

Correspondence to Igor Kovalchuk .

Editor information

Editors and Affiliations

  1. Department of Biological Sciences, University of Lethbridge, Lethbridge, Alberta, Canada

    Igor Kovalchuk

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Bilichak, A., Golubov, A., Kovalchuk, I. (2017). Small RNA Library Preparation and Illumina Sequencing in Plants. In: Kovalchuk, I. (eds) Plant Epigenetics. Methods in Molecular Biology, vol 1456. Humana Press, Boston, MA. https://doi.org/10.1007/978-1-4899-7708-3_15

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  • DOI: https://doi.org/10.1007/978-1-4899-7708-3_15

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  • Publisher Name: Humana Press, Boston, MA

  • Print ISBN: 978-1-4899-7706-9

  • Online ISBN: 978-1-4899-7708-3

  • eBook Packages: Springer Protocols

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