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The Impact of Non-electrical Factors on Electrical Gene Transfer

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Electroporation Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1121))

Abstract

Electrical pulses directly and effectively boost both in vitro and in vivo gene transfer, but this process is greatly affected by non-electrical factors that exist during electroporation. These factors include, but are not limited to, the types of cells or tissues used, property of DNA, DNA formulation, and expressed protein. In this mini-review, we only describe and discuss a summary of DNA properties and selected DNA formulations on gene transfer via electroporation. The properties of DNA were selected for review because a substantial amount of remarkable work has been performed during the past few years but has received less notice than other works, although DNA properties appear to be critical for boosting electroporation delivery. The selected formulations will be covered in this mini-review because we are only interested in the simple formulations that could be used for cell or gene therapy via electroporation. Plus, there was an extensive review of DNA formulations in the first edition of this book. The formulations discussed in this mini-review represent novel developments in recent years and may impact electroporation significantly. These advancements in DNA formulations could prove to be important for gene delivery and disease treatment.

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Author information

Authors and Affiliations

  1. Department of Pediatrics, The University of Texas MD Anderson Cancer Center, Houston, TX, USA

    Jiemiao Hu, Jeffry Cutrera & Shulin Li

  2. Department of Musculoskeletal Oncology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai, China

    Jiemiao Hu & Jeffry Cutrera

Authors
  1. Jiemiao Hu
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  2. Jeffry Cutrera
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  3. Shulin Li
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Editor information

Editors and Affiliations

  1. The University of Texas M.D. Anderson Cancer Center, Houston, Texas, USA

    Shulin Li

  2. The University of Texas M.D. Anderson Cancer Center, Houston, Texas, USA

    Jeffry Cutrera

  3. Old Dominion University Frank Reidy Ctr for Bioelectrics, Norfolk, Virginia, USA

    Richard Heller

  4. IPBS CNR, Toulouse, France

    Justin Teissie

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© 2014 Springer Science+Business Media New York

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Hu, J., Cutrera, J., Li, S. (2014). The Impact of Non-electrical Factors on Electrical Gene Transfer. In: Li, S., Cutrera, J., Heller, R., Teissie, J. (eds) Electroporation Protocols. Methods in Molecular Biology, vol 1121. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4614-9632-8_3

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  • DOI: https://doi.org/10.1007/978-1-4614-9632-8_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4614-9631-1

  • Online ISBN: 978-1-4614-9632-8

  • eBook Packages: Springer Protocols

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