Abstract
Strategies to improve vaccine efficacy are still required. The immunogenicity of DNA vaccines is strongly improved by electroporation (EP). The skin is populated with a wide variety of immune cells, making it an attractive tissue for vaccine delivery. Here we describe a method for the EP-mediated intradermal delivery of DNA vaccines in nonhuman primates, as a model for preclinical development of human vaccines, using noninvasive needleless electrodes.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Fuchs E, Raghavan S (2002) Getting under the skin of epidermal morphogenesis. Nat Rev Genet 3:199–209
Stoitzner P, Green LK, Jung JY et al (2008) Tumor immunotherapy by epicutaneous immunization requires langerhans cells. J Immunol 180:1991–1998
Vogt A, Mahe B, Costagliola D et al (2008) Transcutaneous anti-influenza vaccination promotes both cd4 and cd8 t cell immune responses in humans. J Immunol 180:1482–1489
Frenck RW Jr, Belshe R, Brady RC et al (2011) Comparison of the immunogenicity and safety of a split-virion, inactivated, trivalent influenza vaccine (fluzone(r)) administered by intradermal and intramuscular route in healthy adults. Vaccine 29:5666–5674
Haniffa M, Ginhoux F, Wang XN et al (2009) Differential rates of replacement of human dermal dendritic cells and macrophages during hematopoietic stem cell transplantation. J Exp Med 206:371–385
Andre FM, Gehl J, Sersa G et al (2008) Efficiency of high- and low-voltage pulse combinations for gene electrotransfer in muscle, liver, tumor, and skin. Hum Gene Ther 19: 1261–1271
Hirao LA, Wu L, Khan AS, Satishchandran A, Draghia-Akli R, Weiner DB (2008) Intradermal/subcutaneous immunization by electroporation improves plasmid vaccine delivery and potency in pigs and rhesus macaques. Vaccine 26: 440–448
Vandermeulen G, Staes E, Vanderhaeghen ML, Bureau MF, Scherman D, Preat V (2007) Optimisation of intradermal DNA electrotransfer for immunisation. J Control Release 124:81–87
Malleret B, Maneglier B, Karlsson I et al (2008) Primary infection with simian immunodeficiency virus: plasmacytoid dendritic cell homing to lymph nodes, type i interferon, and immune suppression. Blood 112: 4598–4608
Romain G, van Gulck E, Epaulard O et al (2012) Cd34-derived dendritic cells transfected ex vivo with hiv-gag mrna induce polyfunctional t-cell responses in nonhuman primates. Eur J Immunol 42:2019–2030
Wonderlich ER, Kader M, Wijewardana V, Barratt-Boyes SM (2011) Dissecting the role of dendritic cells in simian immunodeficiency virus infection and aids. Immunol Res 50: 228–234
Martinon F, Kaldma K, Sikut R et al (2009) Persistent immune responses induced by a human immunodeficiency virus DNA vaccine delivered in association with electroporation in the skin of nonhuman primates. Hum Gene Ther 20:1291–1307
Blazevic V, Mannik A, Malm M et al (2006) Induction of human immunodeficiency virus type-1-specific immunity with a novel gene transport unit (gtu)-multihiv DNA vaccine. AIDS Res Hum Retroviruses 22: 667–677
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media New York
About this protocol
Cite this protocol
Adam, L., Le Grand, R., Martinon, F. (2014). Electroporation-Mediated Intradermal Delivery of DNA Vaccines in Nonhuman Primates. In: Li, S., Cutrera, J., Heller, R., Teissie, J. (eds) Electroporation Protocols. Methods in Molecular Biology, vol 1121. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4614-9632-8_27
Download citation
DOI: https://doi.org/10.1007/978-1-4614-9632-8_27
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4614-9631-1
Online ISBN: 978-1-4614-9632-8
eBook Packages: Springer Protocols