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Low-Energy DC Current Ablation in a Mouse Tumor Model

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Electroporation Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1121))

Abstract

Irreversible electroporation has recently been applied to tissue and tumor ablation. This animal model was developed to evaluate optimal parameters for subcutaneous tumor ablation. The immortalized Panc1 tumor cell line was used for generating tumors via subcutaneous injection in mice. Under general anesthesia with complete muscle relaxation, electroporation was performed with the NanoKnife device. Post-procedure tumors were recovered and studied at specified time intervals to assess the efficacy.

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References

  1. Al-Sakere B, Andre F, Bernat C et al (2007) Tumor ablation with irreversible electroporation. PLoS One 2:e1135

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Author information

Authors and Affiliations

  1. Division of Surgical Oncology, Department of Surgery, University of Louisville, Louisville, KY, USA

    Prejesh Philips, Yan Li & Robert C. G. Martin II

Authors
  1. Prejesh Philips
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  2. Yan Li
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  3. Robert C. G. Martin II
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Editor information

Editors and Affiliations

  1. The University of Texas M.D. Anderson Cancer Center, Houston, Texas, USA

    Shulin Li

  2. The University of Texas M.D. Anderson Cancer Center, Houston, Texas, USA

    Jeffry Cutrera

  3. Old Dominion University Frank Reidy Ctr for Bioelectrics, Norfolk, Virginia, USA

    Richard Heller

  4. IPBS CNR, Toulouse, France

    Justin Teissie

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© 2014 Springer Science+Business Media New York

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Philips, P., Li, Y., Martin, R.C.G. (2014). Low-Energy DC Current Ablation in a Mouse Tumor Model. In: Li, S., Cutrera, J., Heller, R., Teissie, J. (eds) Electroporation Protocols. Methods in Molecular Biology, vol 1121. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4614-9632-8_23

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  • DOI: https://doi.org/10.1007/978-1-4614-9632-8_23

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4614-9631-1

  • Online ISBN: 978-1-4614-9632-8

  • eBook Packages: Springer Protocols

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