Abstract
Delivering genes and other materials directly into the tumor tissue causes specifically localized and powerfully enhanced efficacy of treatments; however, these specific effects can cause rapid, drastic changes in the appearance, texture, and consistency of the tumor. These changes complicate clinical response measurements which can confound the results and render recurring treatments difficult to perform and clinical response measurements nearly impossible to obtain accurately. One of the complicating issues is local swelling. Here, we demonstrate how swelling caused by intratumoral gene treatments can confound the clinical results and impede further treatments, and we demonstrate an easy technique to help overcome this potential hurdle.
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References
Escoffre JM, Rols MP (2012) Electrochemotherapy: progress and prospects. Curr Pharm Des 18:3406–3415
Cutrera J, Torrero M, Shiomitsu K, Mauldin N, Li S (2008) Intratumoral bleomycin and il-12 electrochemogenetherapy for treating head and neck tumors in dogs. Methods Mol Biol 423:319–325
Reed SD, Fulmer A, Buckholz J et al (2010) Bleomycin/interleukin-12 electrochemogene therapy for treating naturally occurring spontaneous neoplasms in dogs. Cancer Gene Ther 17:457–464
Torrero MN, Henk WG, Li S (2006) Regression of high-grade malignancy in mice by bleomycin and interleukin-12 electrochemogenetherapy. Clin Cancer Res 12:257–263
Berger RM, Projan SJ, Horwitz SB, Peisach J (1986) The DNA cleavage mechanism of iron-bleomycin. J Biol Chem 261:15955–15959
Chabner BA, Longo DL (2001) Cancer chemotherapy and biotherapy. Lippincott, Williams, and Wilkins, Philadelphia, PA, pp 466–481
Coleman M, Muller S, Quezada A et al (1998) Nonviral interferon alpha gene therapy inhibits growth of established tumors by eliciting a systemic response. Hum Gene Ther 9: 2223–2230
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Cutrera, J., King, G., Jones, P., Gumpel, E., Xia, X., Li, S. (2014). Managing Local Swelling Following Intratumoral Electro-Chemo-Gene Therapy. In: Li, S., Cutrera, J., Heller, R., Teissie, J. (eds) Electroporation Protocols. Methods in Molecular Biology, vol 1121. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4614-9632-8_20
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DOI: https://doi.org/10.1007/978-1-4614-9632-8_20
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4614-9632-8
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