Skip to main content
SpringerLink
Log in

Electroporation-Mediated Delivery of Genes in Rodent Models of Lung Contusion

  • Protocol
  • First Online:
Electroporation Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1121))

  • 2797 Accesses

Abstract

Several of the biological processes involved in the pathogenesis of acute lung injury and acute respiratory distress syndrome after lung contusion are regulated at a genetic and epigenetic level. Thus, strategies to manipulate gene expression in this context are highly desirable not only to elucidate the mechanisms involved but also to look for potential therapies. In the present chapter, we describe mouse and rat models of inducing blunt thoracic injury followed by electroporation-mediated gene delivery to the lung. Electroporation is a highly efficient and easily reproducible technique that allows circumvention of several of lung gene delivery challenges and safety issues present with other forms of lung gene therapy.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Kollmorgen DR, Murray KA, Sullivan JJ, Mone MC, Barton RG (1994) Predictors of mortality in pulmonary contusion. Am J Surg 168:659–663

    Article  CAS  PubMed  Google Scholar 

  2. Miller PR, Croce MA, Kilgo PD, Scott J, Fabian TC (2002) Acute respiratory distress syndrome in blunt trauma: identification of independent risk factors. Am Surg 68:845–850

    PubMed  Google Scholar 

  3. Wu J, Sheng L, Ma Y et al (2008) The analysis of risk factors of impacting mortality rate in severe multiple trauma patients with posttraumatic acute respiratory distress syndrome. Am J Emerg Med 26:419–424

    Article  PubMed  Google Scholar 

  4. ARDS_Network (2000) Ventilation with lower tidal volumes as compared with traditional tidal volumes for acute lung injury and the acute respiratory distress syndrome. The acute respiratory distress syndrome network. N Engl J Med 342:1301–1308

    Article  Google Scholar 

  5. Network ARDS (2000) Ketoconazole for early treatment of acute lung injury and acute respiratory distress syndrome: a randomized controlled trial. The ards network. Jama 283:1995–2002

    Article  Google Scholar 

  6. Blank R, Napolitano LM (2011) Epidemiology of ards and ali. Crit Care Clin 27:439–458

    Article  PubMed  Google Scholar 

  7. Bone RC, Fisher CJ Jr, Clemmer TP, Slotman GJ, Metz CA (1987) Early methylprednisolone treatment for septic syndrome and the adult respiratory distress syndrome. Chest 92:1032–1036

    Article  CAS  PubMed  Google Scholar 

  8. Brower RG, Lanken PN, MacIntyre N et al (2004) Higher versus lower positive end-expiratory pressures in patients with the acute respiratory distress syndrome. N Engl J Med 351:327–336

    Article  PubMed  Google Scholar 

  9. Gallagher DC, Parikh SM, Balonov K et al (2008) Circulating angiopoietin 2 correlates with mortality in a surgical population with acute lung injury/adult respiratory distress syndrome. Shock 29:656–661

    CAS  PubMed  Google Scholar 

  10. Matthay MA, Brower RG, Carson S et al (2011) Randomized, placebo-controlled clinical trial of an aerosolized beta-agonist for treatment of acute lung injury. Am J Respir Crit Care Med 184:561–568

    Article  CAS  PubMed  Google Scholar 

  11. Matthay MA, Zimmerman GA, Esmon C et al (2003) Future research directions in acute lung injury: summary of a national heart, lung, and blood institute working group. Am J Respir Crit Care Med 167:1027–1035

    Article  PubMed  Google Scholar 

  12. Machado-Aranda DA, Suresh MV, Yu B, Raghavendran K (2012) Electroporation-mediated in vivo gene delivery of the na+/k+-atpase pump reduced lung injury in a mouse model of lung contusion. J Trauma Acute Care Surg 72:32–39

    CAS  PubMed Central  PubMed  Google Scholar 

  13. Raghavendran K, Davidson BA, Helinski JD et al (2005) A rat model for isolated bilateral lung contusion from blunt chest trauma. Anesth Analg 101:1482–1489

    Article  PubMed  Google Scholar 

  14. Raghavendran KDB, Helinski JD, Marschke CM, Woytash JA, Notter RH, Knight PR (2004) A new rat model for isolated bilateral lung contusion-reversal of hypoxia by 24 hours. Crit Care Med. Poster presentation at Society for Critical Care Medicine

    Google Scholar 

  15. Suresh MV, Yu B, Machado-Aranda D et al (2012) Role of macrophage chemoattractant protein 1 in acute inflammation following lung contusion. Am. J. Respir. Cell Mol, Biol

    Google Scholar 

  16. Hoth JJ, Hudson WP, Brownlee NA et al (2007) Toll-like receptor 2 participates in the response to lung injury in a murine model of pulmonary contusion. Shock 28:447–452

    Article  CAS  PubMed  Google Scholar 

  17. Hoth JJ, Stitzel JD, Gayzik FS et al (2006) The pathogenesis of pulmonary contusion: an open chest model in the rat. J Trauma 61:32–44, discussion 44–35

    Article  PubMed  Google Scholar 

  18. Hoth JJ, Wells JD, Hiltbold EM, McCall CE, Yoza BK (2011) Mechanism of neutrophil recruitment to the lung after pulmonary contusion. Shock 15:15

    Google Scholar 

  19. Bauer HC, Traweger A, Zweimueller-Mayer J et al (2011) New aspects of the molecular constituents of tissue barriers. J Neural Transm 118:7–21

    Article  CAS  PubMed  Google Scholar 

  20. Lin X, Dean DA (2011) Gene therapy for ali/ards. Crit Care Clin 27:705–718

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  21. Howrylak JA, Dolinay T, Lucht L et al (2009) Discovery of the gene signature for acute lung injury in patients with sepsis. Physiol Genomics 37:133–139

    Article  CAS  PubMed  Google Scholar 

  22. Dean DA, Machado-Aranda D, Blair-Parks K, Yeldandi AV, Young JL (2003) Electroporation as a method for high-level nonviral gene transfer to the lung. Gene Ther 10:1608–1615

    Article  CAS  PubMed  Google Scholar 

  23. Machado-Aranda D, Adir Y, Young JL et al (2005) Gene transfer of the na+, k+-atpase beta1 subunit using electroporation increases lung liquid clearance. Am J Respir Crit Care Med 171:204–211

    Article  PubMed  Google Scholar 

  24. Mutlu GM, Machado-Aranda D, Norton JE et al (2007) Electroporation-mediated gene transfer of the na+, k+-atpase rescues endotoxin-induced lung injury. Am J Respir Crit Care Med 176:582–590

    Article  CAS  PubMed  Google Scholar 

  25. Gatson JW, Liu MM, Abdelfattah K et al (2013) Resveratrol decreases inflammation in the brain of mice with mild traumatic brain injury. J Trauma Acute Care Surg 74:470–475

    Article  CAS  PubMed  Google Scholar 

  26. Mouzon B, Chaytow H, Crynen G et al (2012) Repetitive mild traumatic brain injury in a mouse model produces learning and memory deficits accompanied by histological changes. J Neurotrauma 29:2761–2773

    Article  PubMed  Google Scholar 

  27. Sangiorgi S, De Benedictis A, Protasoni M et al (2013) Early-stage microvascular alterations of a new model of controlled cortical traumatic brain injury: 3d morphological analysis using scanning electron microscopy and corrosion casting. J Neurosurg 118:763–774

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Acute Care Surgery, Department of Surgery, University of Michigan, Ann Arbor, MI, USA

    David Machado-Aranda & Krishnan Raghavendran

Authors
  1. David Machado-Aranda
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Krishnan Raghavendran
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. The University of Texas M.D. Anderson Cancer Center, Houston, Texas, USA

    Shulin Li

  2. The University of Texas M.D. Anderson Cancer Center, Houston, Texas, USA

    Jeffry Cutrera

  3. Old Dominion University Frank Reidy Ctr for Bioelectrics, Norfolk, Virginia, USA

    Richard Heller

  4. IPBS CNR, Toulouse, France

    Justin Teissie

Rights and permissions

Reprints and permissions

Copyright information

© 2014 Springer Science+Business Media New York

About this protocol

Cite this protocol

Machado-Aranda, D., Raghavendran, K. (2014). Electroporation-Mediated Delivery of Genes in Rodent Models of Lung Contusion. In: Li, S., Cutrera, J., Heller, R., Teissie, J. (eds) Electroporation Protocols. Methods in Molecular Biology, vol 1121. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4614-9632-8_18

Download citation

  • DOI: https://doi.org/10.1007/978-1-4614-9632-8_18

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4614-9631-1

  • Online ISBN: 978-1-4614-9632-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation