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Electroporation Protocols pp 165–177Cite as

DNA Delivery in Adult Mouse Eyes: An Update with Corneal Outcomes

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1121))

Abstract

Ocular injection (intravitreal, subretinal, or into the anterior space) is an efficient approach to deliver many classes of drugs, cells, and other treatments to various cell types of the eye. In particular, subretinal injection is efficient since delivered agents accumulate as there is no dilution due to transport processes or diffusion and the volume of the interphotoreceptor space (IPS) is minimal (10–20 μl in the human eye, less than 1 μl in the mouse eye). We previously reported methods using subretinal injection and electroporation to deliver DNA to photoreceptor and retinal pigment epithelium cells in retinas of live mice (Johnson et al., 14:2211–2226; Nickerson et al. 884:53–69, 2012; Andrieu-Soler et al. 13:692–706, 2007). Here we detail further optimization of that approach and additionally report its use in delivering DNA expression plasmids to the corneal endothelium.

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Acknowledgments

This work was supported by the National Eye Institute (R01EY016470, R01EY021592, R01EY014026, P30EY006360, R24EY017045, T32EY007092), an unrestricted grant to the Department of Ophthalmology at Emory University from Research to Prevent Blindness, Inc., and the Katz Foundation.

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Authors and Affiliations

  1. Department of Ophthalmology, Emory University, Atlanta, GA, USA

    John M. Nickerson, Shannon E. Getz, Jana T. Sellers, Micah A. Chrenek, Penny Goodman, Christiana J. Bernal & Jeffrey H. Boatright

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  1. John M. Nickerson
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  2. Shannon E. Getz
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  3. Jana T. Sellers
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  4. Micah A. Chrenek
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  5. Penny Goodman
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  6. Christiana J. Bernal
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  7. Jeffrey H. Boatright
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Editor information

Editors and Affiliations

  1. The University of Texas M.D. Anderson Cancer Center, Houston, Texas, USA

    Shulin Li

  2. The University of Texas M.D. Anderson Cancer Center, Houston, Texas, USA

    Jeffry Cutrera

  3. Old Dominion University Frank Reidy Ctr for Bioelectrics, Norfolk, Virginia, USA

    Richard Heller

  4. IPBS CNR, Toulouse, France

    Justin Teissie

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Nickerson, J.M. et al. (2014). DNA Delivery in Adult Mouse Eyes: An Update with Corneal Outcomes. In: Li, S., Cutrera, J., Heller, R., Teissie, J. (eds) Electroporation Protocols. Methods in Molecular Biology, vol 1121. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4614-9632-8_15

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  • DOI: https://doi.org/10.1007/978-1-4614-9632-8_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4614-9631-1

  • Online ISBN: 978-1-4614-9632-8

  • eBook Packages: Springer Protocols

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