Abstract
Here, we outline a method for surface-mediated transfection of small interfering RNA (siRNA) using electroporation. Cells are cultured on an electrode carrying siRNA. After the cells are adhered on the electrode surface, an electric pulse is applied to release siRNA from the surface and transfect it into the cells. A siRNA electroporation microarray is also presented for high-throughput screening of the functions of many siRNAs.
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Fujimoto, H., Iwata, H. (2014). Substrate-Mediated, High-Efficiency siRNA Electroporation. In: Li, S., Cutrera, J., Heller, R., Teissie, J. (eds) Electroporation Protocols. Methods in Molecular Biology, vol 1121. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4614-9632-8_12
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DOI: https://doi.org/10.1007/978-1-4614-9632-8_12
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4614-9631-1
Online ISBN: 978-1-4614-9632-8
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