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Molecular Typing Methods for Minor Histocompatibility Antigens

  • Eric Spierings
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1109)

Abstract

Minor histocompatibility (H) antigen mismatching leads to clinically relevant alloimmune reactivity. Depending on the tissue expression pattern of the involved minor H antigens, the immune response may either cause graft-versus-host disease and a graft-versus-tumor effect or lead to only a graft-versus-leukemia effect. Thus, identification of recipient–donor pairs with minor H antigen mismatches has clinical importance. This chapter describes molecular typing methods for molecular typing of minor H antigens.

Key words

Minor histocompatibility antigens Transplantation Stem cells Solid organ Graft-versus-host Graft-versus-tumor Graft rejection Genotyping 

Notes

Acknowledgments

Talitha de Hoop and Walter van Ginkel, University Medical Center Utrecht, are acknowledged for developing and validating the SBT protocols; Jos Pool and Jos Drabbels, Leiden University Medical Center, for developing and validating the uniform PCR-SSP protocols; and Erik Rozemuller, GenDx BV, Utrecht, for the implementation of automated allele assignment for minor H antigens after SBT in SBTEngine©.

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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  • Eric Spierings
    • 1
  1. 1.Laboratory for Translational ImmunologyUniversity Medical Center UtrechtUtrechtThe Netherlands

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