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Bone Marrow and Stem Cell Transplantation pp 47–63Cite as

Isolation and Characterization of Mesenchymal Stem Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1109))

Abstract

Mesenchymal stem cells (MSCs) have drawn great interest in the field of regenerative medicine, for cell replacement, immunomodulatory, and gene therapies. It has been shown that these multipotent stromal cells can be isolated from tissues such as bone marrow, adipose tissue, trimester amniotic tissue, umbilical cord blood, and deciduous teeth and can be expanded in adherent culture. They have the capacity to differentiate into cells of the connective tissue lineages in vitro and contribute to tissue parenchyma in vivo. However, proper in vitro manipulation of MSCs is a key issue to reveal a potential therapeutic benefit following transplantation into the patients. This chapter summarizes some of the essential protocols and assays used at our laboratory for the isolation, culture, differentiation, and characterization of mesenchymal stem cells from the bone marrow and adipose tissue.

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Acknowledgments

YME acknowledges the support of The Turkish Academy of Sciences (TÜBA), Ankara.

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Authors and Affiliations

  1. Faculty of Sciences and Stem Cell Institute, Ankara University, Ankara, Turkey

    Sedat Odabas, A. Eser Elçin & Y. Murat Elçin

Authors
  1. Sedat Odabas
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  2. A. Eser Elçin
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  3. Y. Murat Elçin
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Editor information

Editors and Affiliations

  1. Department of Hematology, Ankara University, Ankara, Turkey

    Meral Beksaç

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© 2014 Springer Science+Business Media, New York

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Odabas, S., Elçin, A.E., Elçin, Y.M. (2014). Isolation and Characterization of Mesenchymal Stem Cells. In: Beksaç, M. (eds) Bone Marrow and Stem Cell Transplantation. Methods in Molecular Biology, vol 1109. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4614-9437-9_3

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  • DOI: https://doi.org/10.1007/978-1-4614-9437-9_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4614-9436-2

  • Online ISBN: 978-1-4614-9437-9

  • eBook Packages: Springer Protocols

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