Abstract
Growing tips of plants harbor a set of stem cells in structures called shoot apical meristems (SAMs) which provide cells for development of aboveground biomass. Despite a periodic differentiation of stem cell progenitors into leaves, the stem cell pool remains constant over time. Genetic analysis has revealed molecular pathways involved in stem-cell specification, cell division patterns, and organ differentiation. Stem cells within SAMs are few in number, which imposes a limitation to the experimental approaches that can be used for deciphering the gene regulatory networks that underlie cell fate transitions. Here, I provide detailed experimental protocols for the protoplasting and subsequent purification through cell sorting of SAM cells, which allows genome-wide analyses of gene expression patterns at a single cell-type resolution.
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Acknowledgements
Our genomics work is being carried out at the core facility of center for plant cell biology (CEPCEB) and institute of integrative genome biology (IIGB), University of California, Riverside. This work is funded by National Science Foundation grant (IOS-1147250) to G.V.R.
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Reddy, G.V. (2014). Fluorescence Activated Cell Sorting of Shoot Apical Meristem Cell Types. In: Riechmann, J., Wellmer, F. (eds) Flower Development. Methods in Molecular Biology, vol 1110. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4614-9408-9_17
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DOI: https://doi.org/10.1007/978-1-4614-9408-9_17
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