Skip to main content
SpringerLink
Log in

Microscopic Analysis of Arabidopsis Ovules

  • Protocol
  • First Online:
Flower Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1110))

Abstract

Ovules are the major female reproductive organs in higher plants. Furthermore, ovules of Arabidopsis thaliana are successfully used as model system to study plant organogenesis. Here we describe two microscopic techniques to analyze ovule development in Arabidopsis. Both methods involve fixed specimens and allow rapid, easy, and reproducible morphological comparisons between wild-type and mutant ovule development.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Chevalier D, Sieber P, Schneitz K (2002) The genetic and molecular control of ovule development. In: O' Neill SD, Roberts JA (eds) Plant Reproduction. Sheffield Academic Press, Sheffield

    Google Scholar 

  2. Colombo L, Battaglia R, Kater MM (2008) Arabidopsis ovule development and its evolutionary conservation. Trends Plant Sci 13:444–450

    Article  CAS  PubMed  Google Scholar 

  3. Kelley DR, Gasser CS (2009) Ovule development: genetic trends and evolutionary considerations. Sex Plant Reprod 22:229–234

    Article  PubMed Central  PubMed  Google Scholar 

  4. Shi D, Yang W (2011) Ovule development in Arabidopsis: progress and challenge. Curr Opin Plant Biol 14:74–80

    Article  CAS  PubMed  Google Scholar 

  5. Enugutti B, Kirchhelle C, Schneitz K (2012) On the genetic control of planar growth during tissue morphogenesis in plants. Protoplasma 250:651–661

    Article  PubMed  Google Scholar 

  6. Schneitz K, Hülskamp M, Pruitt RE (1995) Wild-type ovule development in Arabidopsis thaliana: a light microscope study of cleared whole-mount tissue. Plant J 7:731–749

    Article  Google Scholar 

  7. Haseloff J (2003) Old botanical techniques for new microscopes. Biotechniques 34:1174–1182

    CAS  PubMed  Google Scholar 

  8. Truernit E, Bauby H, Dubreucq B, Grandjean O, Runions J, Barthelemy J, Palauqui JC (2008) High-resolution whole-mount imaging of three-dimensional tissue organization and gene expression enables the study of phloem development and structure in Arabidopsis. Plant Cell 20:1494–1503

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  9. Christensen CA, King EJ, Jordan JR, Drews GN (1997) Megagametogenesis in Arabidopsis wild type and the Gf mutant. Sex Plant Reprod 10:49–64

    Article  Google Scholar 

  10. Running MP, Clark SE, Meyerowitz EM (1995) Confocal microscopy of the shoot apex. Methods Cell Biol 49:217–229

    Article  CAS  PubMed  Google Scholar 

  11. Enugutti B, Oelschner M, Schneitz K (2013) Microscopic analysis of ovule development in Arabidopsis thaliana. Methods Mol Biol 959:127–136

    Article  PubMed  Google Scholar 

  12. Schneitz K, Hülskamp M, Kopczak SD, Pruitt RE (1997) Dissection of sexual organ ontogenesis: a genetic analysis of ovule development in Arabidopsis thaliana. Development 124:1367–1376

    CAS  PubMed  Google Scholar 

  13. Smyth DR, Bowman JL, Meyerowitz EM (1990) Early flower development in Arabidopsis. Plant Cell 2:755–767

    CAS  PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgment

Our work is supported by grants from the Deutsche Forschungsgemeinschaft (German Research Council) to Kay Schneitz.

Author information

Authors and Affiliations

  1. Entwicklungsbiologie der Pflanzen, Wissenschaftszentrum Weihenstephan, Technische Universität München, Freising, Germany

    Balaji Enugutti & Kay Schneitz

Authors
  1. Balaji Enugutti
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kay Schneitz
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kay Schneitz .

Editor information

Editors and Affiliations

  1. Center for Research in Agricultural Genomics, Barcelona, Spain

    José Luis Riechmann

  2. Trinity College Dublin, Ireland

    Frank Wellmer

Rights and permissions

Reprints and permissions

Copyright information

© 2014 Springer Science+Business Media, New York

About this protocol

Cite this protocol

Enugutti, B., Schneitz, K. (2014). Microscopic Analysis of Arabidopsis Ovules. In: Riechmann, J., Wellmer, F. (eds) Flower Development. Methods in Molecular Biology, vol 1110. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4614-9408-9_12

Download citation

  • DOI: https://doi.org/10.1007/978-1-4614-9408-9_12

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4614-9407-2

  • Online ISBN: 978-1-4614-9408-9

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation