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Flower Development pp 217–230Cite as

Molecular Cell Biology of Male Meiotic Chromosomes and Isolation of Male Meiocytes in Arabidopsis thaliana

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1110))

Abstract

Plants typically produce numerous flowers whose meiotic chromosomes are relatively easy to observe, making them excellent structures for studying the cellular processes underlying meiosis. In recent years, breakthroughs in light and electron microscopic technologies for small chromosomes, combined with molecular genetic methods, have resulted in major advances in the understanding of meiosis in the model plant Arabidopsis thaliana. In this chapter, we summarize protocols for basic cytology, fluorescence in situ hybridization, immunofluorescence, electron microscopy, and isolation of male meiocytes for the analysis of Arabidopsis meiosis.

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Acknowledgment

We thank Dr. Zaibao Zhang and Jiyue Huang for helpful suggestions and comments. This work was supported by grants from the Ministry of Sciences and Technology of China (2011CB944600).

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Authors and Affiliations

  1. State Key Laboratory of Genetic Engineering, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, China

    Yingxiang Wang, Zhihao Cheng & Hong Ma

  2. Department of Biology, The Pennsylvania State University, University Park, PA, USA

    Pingli Lu

  3. The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, USA

    Pingli Lu

  4. Department of Plant Genetics, Institute of Experimental Biology at the Estonian Agricultural University, Harku, Harju maakond, Estonia

    Ljudmilla Timofejeva

  5. Center for Evolutionary Biology, School of Life Sciences, Fudan University, Shanghai, China

    Hong Ma

Authors
  1. Yingxiang Wang
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  2. Zhihao Cheng
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  3. Pingli Lu
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  4. Ljudmilla Timofejeva
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  5. Hong Ma
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Corresponding author

Correspondence to Hong Ma .

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Editors and Affiliations

  1. Center for Research in Agricultural Genomics, Barcelona, Spain

    José Luis Riechmann

  2. Trinity College Dublin, Ireland

    Frank Wellmer

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Wang, Y., Cheng, Z., Lu, P., Timofejeva, L., Ma, H. (2014). Molecular Cell Biology of Male Meiotic Chromosomes and Isolation of Male Meiocytes in Arabidopsis thaliana . In: Riechmann, J., Wellmer, F. (eds) Flower Development. Methods in Molecular Biology, vol 1110. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4614-9408-9_10

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  • DOI: https://doi.org/10.1007/978-1-4614-9408-9_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4614-9407-2

  • Online ISBN: 978-1-4614-9408-9

  • eBook Packages: Springer Protocols

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