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Isolation of Mitochondria from Brain Tissue and Cells

  • Changhai TianEmail author
  • Jialin Zheng
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Mitochondria are one of intracellular organelles acting as the main energy providers of the cell. Increasing evidences have demonstrated that mitochondrial dysfunctions in respiratory chain function, oxidative stress, dynamics (shape, size, fission-fusion, distribution, movement, etc.), calcium-handling capacities, and protein mutation play a central pathogenic role in neurodegenerative diseases such as Parkinson’s disease, Huntington’s disease, amyotrophic lateral sclerosis, and Alzheimer’s disease. Protocols developed for the isolation of pure and intact mitochondria will contribute considerably to the understanding of the pathogenic mechanisms of neurodegenerative disorders.

Keywords

Mitochondria Brain tissues Isolation Reactive oxygen species Neurodegenerative disorders 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Laboratory of Neuroimmunology and Regenerative Therapy, Department of Pharmacology and Experimental NeuroscienceUniversity of Nebraska Medical CenterOmahaUSA

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