Advertisement

Isolation and Culture of Neural Stem/Progenitor Cells

  • Hui PengEmail author
  • Qiang Chen
  • Jialin Zheng
Protocol
  • 3.9k Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Neural stem cells, with the capacity of self-renewal and abilities to differentiate into the major cell types of the brain, exist in the developing and adult rodent central nervous system. These cells can be grown in vitro for long periods of time while retaining the capacity to differentiate into neurons, astrocytes, and oligodendrocytes. In this chapter, we describe the steps in detail to isolate and expand neural stem cells in the form of neurospheres from tissue dissections of the embryonic mouse brain. Procedures for the long-term passage of neurospheres, the cryopreservation of neurospheres, and their differentiation are also provided. Although the methodology seems simple, strict adherence to the procedures described is required in order to achieve reliable and consistent results.

Keywords

Neural stem cell Neural progenitor cells Neurosphere Differentiation 

References

  1. Davis AA, Temple S (1994) A self-renewing multipotential stem cell in embryonic rat cerebral cortex. Nature 372:263–266CrossRefPubMedGoogle Scholar
  2. Doetsch F, Scharff C (2001) Challenges for brain repair: insights from adult neurogenesis in birds and mammals. Brain Behav Evol 58:306–322CrossRefPubMedGoogle Scholar
  3. Gage FH (2000) Mammalian neural stem cells. Science 287:1433–1438CrossRefPubMedGoogle Scholar
  4. Heldmann U, Mine Y, Kokaia Z, Ekdahl CT, Lindvall O (2011) Selective depletion of Mac-1-expressing microglia in rat subventricular zone does not alter neurogenic response early after stroke. Exp Neurol 229:391–398CrossRefPubMedGoogle Scholar
  5. Jagasia R, Song H, Gage FH, Lie DC (2006) New regulators in adult neurogenesis and their potential role for repair. Trends Mol Med 12:400–405CrossRefPubMedGoogle Scholar
  6. Limke TL, Rao MS (2002) Neural stem cells in aging and disease. J Cell Mol Med 6:475–96Google Scholar
  7. Reynolds BA, Weiss S (1992) Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science 255:1707–1710CrossRefPubMedGoogle Scholar
  8. Seri B, Garcia-Verdugo JM, McEwen BS, Alvarez-Buylla A (2001) Astrocytes give rise to new neurons in the adult mammalian hippocampus. J Neurosci 21:7153–7160PubMedGoogle Scholar
  9. Weiss S, Reynolds BA, Vescovi AL, Morshead C, Craig CG, van der Kooy D (1996) Is there a neural stem cell in the mammalian forebrain? Trends Neurosci 19:387–393CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.University of Nebraska Medical CenterOmahaUSA

Personalised recommendations