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Stereological Analysis

  • Kristi M. Anderson
  • Adam M. Szlachetka
  • R. Lee MosleyEmail author
Protocol
  • 3.9k Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The use of stereology for quantitative estimates of biological features is becoming commonplace in modern laboratories. Over the past decades, stereology has evolved to keep pace with the ever-changing field of neuroscience. It has moved from its early applications for studying the geologic composition of rocks to an invaluable method to detect both small- and large-scale changes in the complex central nervous system. Recent developments in technology have made the practice highly efficient and accurate while providing a strategy to remove assumptions and investigator biases that lead to inaccurate numerical estimations. This chapter focuses on the use of stereology in neuroscience and provides evidence that validates the results generated via stereological analysis. The reader is introduced to the many applications of stereology in the laboratory including the quantification of neuron populations, measurement of dendrite and axon lengths, analysis of surface area of non-symmetrical shapes, and overall connectivity in the brain. This chapter provides systematic examples for the performance of a stereological study and is presented in a manner allowing the use of this chapter as a manual directly translatable for the neuroscience laboratory.

Keywords

Stereology Designed-based quantitation CNS neurons Fractionators Disector 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Kristi M. Anderson
    • 1
  • Adam M. Szlachetka
    • 1
  • R. Lee Mosley
    • 1
    Email author
  1. 1.Department of Pharmacology and Experimental Neuroscience, Center for Neurodegenerative DisordersUniversity of Nebraska Medical CenterOmahaUSA

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