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Humanized Mice

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Current Laboratory Methods in Neuroscience Research

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Abstract

Human-specific viral infections of CNS require the presence of human host cells. For modeling of HIV-1-associated neurocognitive diseases, the presence of human cells of macrophage lineage that support viral replication is necessary. This process is recognized as HIV-1 encephalitis and mirrored in immunodeficient mice by transplantation of HIV-1-infected human macrophages in deep brain nuclei. However, the development of minor cognitive/motor decline can be observed in the absence of encephalitis. These types of pathology could be imitated in chronically infected animals. The stable engraftment of human hematopoietic stem cells and development of human immune system facilitated establishment of chronic HIV-1 infection. The chapter describes both approaches to mimic human HIV-1-associated pathologies, requirements for mouse strain background, and morphologic evaluation of brain pathology.

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Authors and Affiliations

  1. Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, 985880 Nebraska Medical Center, Omaha, NE, 68198-5880, USA

    Larisa Y. Poluektova & Edward Makarov

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  1. Larisa Y. Poluektova
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  2. Edward Makarov
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Correspondence to Larisa Y. Poluektova .

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  1. Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha, NE, USA

    Huangui Xiong  & Howard E. Gendelman  & 

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Poluektova, L.Y., Makarov, E. (2014). Humanized Mice. In: Xiong, H., Gendelman, H.E. (eds) Current Laboratory Methods in Neuroscience Research. Springer Protocols Handbooks. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-8794-4_33

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  • DOI: https://doi.org/10.1007/978-1-4614-8794-4_33

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