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Humanized Mice

  • Larisa Y. PoluektovaEmail author
  • Edward Makarov
Protocol
  • 3.9k Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Human-specific viral infections of CNS require the presence of human host cells. For modeling of HIV-1-associated neurocognitive diseases, the presence of human cells of macrophage lineage that support viral replication is necessary. This process is recognized as HIV-1 encephalitis and mirrored in immunodeficient mice by transplantation of HIV-1-infected human macrophages in deep brain nuclei. However, the development of minor cognitive/motor decline can be observed in the absence of encephalitis. These types of pathology could be imitated in chronically infected animals. The stable engraftment of human hematopoietic stem cells and development of human immune system facilitated establishment of chronic HIV-1 infection. The chapter describes both approaches to mimic human HIV-1-associated pathologies, requirements for mouse strain background, and morphologic evaluation of brain pathology.

Keywords

Immunocompromised mice scid mutation Macrophages Hematopoietic stem cells HIV-1 Encephalitis Neuroinflammation Microglia Peripheral blood lymphocytes 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of Pharmacology and Experimental NeuroscienceUniversity of Nebraska Medical CenterOmahaUSA

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