Abstract
DNA microarray technology is a useful tool to further our understanding of underlying biological pathways governing the behavior of cells and tissues. In fact, microarrays have evolved over the last 10 years or so to become one of the major technologies utilized in biomedical research. This chapter will provide a brief history of the development of DNA microarray technology, a brief survey of widely accepted microarray platforms, and then expand upon critical components of DNA microarray experimentation necessary to ensure optimal outcomes in a given microarray experiment. Topics covered will include the necessity of a good initial experimental design, quality control issues with respect to input RNA or DNA, appropriate use of biological replicates relevant to a given biological question, and analysis strategies employed to extract meaningful results from a microarray experiment.
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Eudy, J.D., Smith, L. (2014). DNA Microarrays as a Tool for Neurosciences Research. In: Xiong, H., Gendelman, H.E. (eds) Current Laboratory Methods in Neuroscience Research. Springer Protocols Handbooks. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-8794-4_29
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DOI: https://doi.org/10.1007/978-1-4614-8794-4_29
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