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DNA Microarrays as a Tool for Neurosciences Research

  • James D. EudyEmail author
  • Lynette Smith
Protocol
  • 3.9k Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

DNA microarray technology is a useful tool to further our understanding of underlying biological pathways governing the behavior of cells and tissues. In fact, microarrays have evolved over the last 10 years or so to become one of the major technologies utilized in biomedical research. This chapter will provide a brief history of the development of DNA microarray technology, a brief survey of widely accepted microarray platforms, and then expand upon critical components of DNA microarray experimentation necessary to ensure optimal outcomes in a given microarray experiment. Topics covered will include the necessity of a good initial experimental design, quality control issues with respect to input RNA or DNA, appropriate use of biological replicates relevant to a given biological question, and analysis strategies employed to extract meaningful results from a microarray experiment.

Keywords

DNA microarray Gene expression RNA DNA Normalization Hybridization Analysis 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of Genetics, Cell Biology, and AnatomyUniversity of Nebraska Medical CenterOmahaUSA
  2. 2.Department of Biostatistics, College of Public HealthUniversity of Nebraska Medical CenterOmahaUSA

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