Techniques in Immunohistochemistry and Immunocytochemistry

  • Yang Yuan
  • Jyothi ArikkathEmail author
Part of the Springer Protocols Handbooks book series (SPH)


Immunohistochemistry and immunocytochemistry are emerging as powerful tools to supplement the knowledge obtained by routine histological techniques. These have been significantly aided by technical advances in microscopy. The combination of immunolabeling techniques and microscopy now allows detailed insights into tissue and cellular architecture and is proving to be extremely valuable for understanding the micro architecture of the brain in health and disease. In this chapter, we discuss some of the considerations and techniques for immunohistochemistry of the brain and dissociated, cultured neurons from the hippocampus and cortex.


Immunolabeling Immunocytochemistry Immunohistochemistry Antibody Fluorescence microscopy 


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Developmental Neuroscience, Munroe-Meyer InstituteUniversity of Nebraska Medical CenterOmahaUSA

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