Advertisement

Techniques in Immunohistochemistry and Immunocytochemistry

  • Yang Yuan
  • Jyothi ArikkathEmail author
Protocol
  • 3.9k Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Immunohistochemistry and immunocytochemistry are emerging as powerful tools to supplement the knowledge obtained by routine histological techniques. These have been significantly aided by technical advances in microscopy. The combination of immunolabeling techniques and microscopy now allows detailed insights into tissue and cellular architecture and is proving to be extremely valuable for understanding the micro architecture of the brain in health and disease. In this chapter, we discuss some of the considerations and techniques for immunohistochemistry of the brain and dissociated, cultured neurons from the hippocampus and cortex.

Keywords

Immunolabeling Immunocytochemistry Immunohistochemistry Antibody Fluorescence microscopy 

References

  1. Ding JB, Takasaki KT, Sabatini BL (2009) Supraresolution imaging in brain slices using stimulated-emission depletion two-photon laser scanning microscopy. Neuron 63(4):429–437CrossRefPubMedPubMedCentralGoogle Scholar
  2. Emoto K (2011) Dendrite remodeling in development and disease. Dev Growth Differ 53(3):277–286CrossRefPubMedGoogle Scholar
  3. Kaech S, Banker G (2006) Culturing hippocampal neurons. Nat Protoc 1(5):2406–2415CrossRefPubMedGoogle Scholar
  4. Micheva KD, Smith SJ (2007) Array tomography: a new tool for imaging the molecular architecture and ultrastructure of neural circuits. Neuron 55(1):25–36CrossRefPubMedPubMedCentralGoogle Scholar
  5. Ness JM, Akhtar RS, Latham CB, Roth KA (2003) Combined tyramide signal amplification and quantum dots for sensitive and photostable immunofluorescence detection. J Histochem Cytochem 51(8):981–987CrossRefPubMedGoogle Scholar
  6. Penzes P, Cahill ME, Jones KA, VanLeeuwen JE, Woolfrey KM (2011) Dendritic spine pathology in neuropsychiatric disorders. Nat Neurosci 14(3):285–293CrossRefPubMedPubMedCentralGoogle Scholar
  7. Rust MJ, Bates M, Zhuang X (2006) Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). Nat Methods 3(10):793–795CrossRefPubMedPubMedCentralGoogle Scholar
  8. Taraska JW, Zagotta WN (2010) Fluorescence applications in molecular neurobiology. Neuron 66(2):170–189CrossRefPubMedPubMedCentralGoogle Scholar
  9. Wang G, Achim CL, Hamilton RL, Wiley CA, Soontornniyomkij V (1999) Tyramide signal amplification method in multiple-label immunofluorescence confocal microscopy. Methods 18(4):459–464CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Developmental Neuroscience, Munroe-Meyer InstituteUniversity of Nebraska Medical CenterOmahaUSA

Personalised recommendations