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“Blind” Patch-Clamp Recordings from Rodent Hippocampal Slices

  • Jianxun Xia
  • Huangui XiongEmail author
Protocol
  • 3.9k Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Whole-cell patch-clamp recording technique has enabled electrophysiologists to study synaptic transmission and membrane properties of neurons in an isolated preparation as an integral part of synaptic and cellular physiology. Compared with visually guided patch-clamp recording, the “blind” method does not require expensive experimental equipment and is easy to develop. This chapter describes “hands-on” procedures of “blind” patch-clamp recording on rodent hippocampal slices, followed by a discussion of the advantages and potential pitfalls with possible solutions.

Keywords

Brain slices Hippocampus Electrophysiology Whole-cell patch clamp 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of Pharmacology and Experimental NeuroscienceUniversity of Nebraska Medical Center, Durham Research CenterOmahaUSA

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