“Blind” Patch-Clamp Recordings from Rodent Hippocampal Slices
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Whole-cell patch-clamp recording technique has enabled electrophysiologists to study synaptic transmission and membrane properties of neurons in an isolated preparation as an integral part of synaptic and cellular physiology. Compared with visually guided patch-clamp recording, the “blind” method does not require expensive experimental equipment and is easy to develop. This chapter describes “hands-on” procedures of “blind” patch-clamp recording on rodent hippocampal slices, followed by a discussion of the advantages and potential pitfalls with possible solutions.