“Blind” Patch-Clamp Recordings from Rodent Hippocampal Slices

  • Jianxun Xia
  • Huangui XiongEmail author
Part of the Springer Protocols Handbooks book series (SPH)


Whole-cell patch-clamp recording technique has enabled electrophysiologists to study synaptic transmission and membrane properties of neurons in an isolated preparation as an integral part of synaptic and cellular physiology. Compared with visually guided patch-clamp recording, the “blind” method does not require expensive experimental equipment and is easy to develop. This chapter describes “hands-on” procedures of “blind” patch-clamp recording on rodent hippocampal slices, followed by a discussion of the advantages and potential pitfalls with possible solutions.


Brain slices Hippocampus Electrophysiology Whole-cell patch clamp 


  1. Blanton MG, Lo Turco JJ, Kriegstein AR (1989) Whole cell recording from neurons in slices of reptilian and mammalian cerebral cortex. J Neurosci Methods 30:203–210CrossRefPubMedGoogle Scholar
  2. Deng P, Xu ZC (2012) Whole-cell patch-clamp recordings on spinal cord slices. Methods Mol Biol 851:65–72CrossRefPubMedGoogle Scholar
  3. Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ (1981) Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch 391:85–100CrossRefPubMedGoogle Scholar
  4. Liu ZW, Li LJ, Liu CG (2001) Blind patch clamp whole-cell recording technique for neurons in hippocampal slices. Sheng Li Xue Bao 53:405–408PubMedGoogle Scholar
  5. Marty A, Neher E (1983) Tight-seal whole-cell recoding. In: Sakmann B, Neher E (eds) Single-channel recording. Plenum, New York, NY, pp 107–122CrossRefGoogle Scholar
  6. Okada Y (2012) Patch clamp techniques: from beginning to advanced protocols. Springer, TokyoCrossRefGoogle Scholar
  7. Walz W (2007) Patch-clamp analysis: advanced techniques, 2nd edn. Humana, TotowaCrossRefGoogle Scholar
  8. Windhorst U, Johansson H (1999) Modern techniques in neuroscience research. Springer, BerlinCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of Pharmacology and Experimental NeuroscienceUniversity of Nebraska Medical Center, Durham Research CenterOmahaUSA

Personalised recommendations