Abstract
This chapter provides a general introduction of classifications and applications of the extracellular electrophysiology recording techniques. Among those, recordings of field potentials and compound action potentials on hippocampal brain slices are given as an example to illustrate detailed methods for extracellular recording ex vivo, due to the extensive usage of the rodent hippocampal slices in neuroscience studies. The equipment required for performing the basic experiment is listed in text. The relevant concepts of field excitatory postsynaptic potential (fEPSP), population spikes (PS), and presynaptic volleys (PSV) are described herein. Accordingly, the protocols for recording fEPSP and PS are introduced, along with the analysis and interpretation of the data. The typical results of the recorded fEPSP and PS as waveform figures, as well as the summary of the relevant data as plotting figures, are also illustrated.
In addition, single unit recordings in the cat and rat medullar oblongata are used as examples of an in vivo extracellular recording. The notions and principles of orthodromic- and antidromic- evoked single unit discharge and spontaneous unit discharges are introduced and the protocols for stimulation and recording are provided.
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Bartho P, Hirase H, Monconduit L, Zugaro M, Harris KD, Buzsaki G (2004) Characterization of neocortical principal cells and interneurons by network interactions and extracellular features. J Neurophysiol 92:600–608
Du J, Riedel-Kruse IH, Nawroth JC, Roukes ML, Laurent G, Masmanidis SC (2009) High-resolution three-dimensional extracellular recording of neuronal activity with microfabricated electrode arrays. J Neurophysiol 101:1671–1678
Erickson J, Tooker A, Tai YC, Pine J (2008) Caged neuron MEA: a system for long-term investigation of cultured neural network connectivity. J Neurosci Methods 175:1–16
Field KJ, White WJ, Lang CM (1993) Anaesthetic effects of chloral hydrate, pentobarbitone and urethane in adult male rats. Lab Anim 27:258–269
Gholmieh G, Courellis S, Dimoka A, Wills JD, LaCoss J, Granacki JJ, Marmarelis V, Berger T (2004) An algorithm for real-time extraction of population EPSP and population spike amplitudes from hippocampal field potential recordings. J Neurosci Methods 136:111–121
Gray CM, Maldonado PE, Wilson M, McNaughton B (1995) Tetrodes markedly improve the reliability and yield of multiple single-unit isolation from multi-unit recordings in cat striate cortex. J Neurosci Methods 63:43–54
Henze DA, Borhegyi Z, Csicsvari J, Mamiya A, Harris KD, Buzsaki G (2000) Intracellular features predicted by extracellular recordings in the hippocampus in vivo. J Neurophysiol 84:390–400
Juergens E, Guettler A, Eckhorn R (1999) Visual stimulation elicits locked and induced gamma oscillations in monkey intracortical- and EEG-potentials, but not in human EEG. Exp Brain Res 129:247–259
Kauer JA (1999) Blockade of hippocampal long-term potentiation by sustained tetanic stimulation near the recording site. J Neurophysiol 81:940–944
Legatt AD, Arezzo J, Vaughan HG Jr (1980) Averaged multiple unit activity as an estimate of phasic changes in local neuronal activity: effects of volume-conducted potentials. J Neurosci Methods 2:203–217
Li S, Jin M, Koeglsperger T, Shepardson NE, Shankar GM, Selkoe DJ (2011) Soluble Abeta oligomers inhibit long-term potentiation through a mechanism involving excessive activation of extrasynaptic NR2B-containing NMDA receptors. J Neurosci 31:6627–6638
Luo PF, Wang BR, Peng ZZ, Li JS (1991) Morphological characteristics and terminating patterns of masseteric neurons of the mesencephalic trigeminal nucleus in the rat: an intracellular horseradish peroxidase labeling study. J Comp Neurol 303:286–299
Luo P, Zhang J, Yang R, Pendlebury W (2006) Neuronal circuitry and synaptic organization of trigeminal proprioceptive afferents mediating tongue movement and jaw-tongue coordination via hypoglossal premotor neurons. Eur J Neurosci 23:3269–3283
Mathis DM, Furman JL, Norris CM (2011) Preparation of acute hippocampal slices from rats and transgenic mice for the study of synaptic alterations during aging and amyloid pathology. J Vis Exp (49), e2330, doi:10.3791/2330
Mendell LM (1966) Physiological properties of unmyelinated fiber projection to the spinal cord. Exp Neurol 16:316–332
Myers FB, Abilez OJ, Zarins CK, Lee LP (2011) Stimulation and artifact-free extracellular electrophysiological recording of cells in suspension. Conf Proc IEEE Eng Med Biol Soc 2011:4030–4033
Paxinos G, Watson C (eds) (1998) The rat brain in stereotaxic coordinates. Academic, San Diego
Ro JY, Capra NF (1999a) Evidence for subnucleus interpolaris in craniofacial muscle pain mechanisms demonstrated by intramuscular injections with hypertonic saline. Brain Res 842:166–183
Ro JY, Capra NF (1999b) Physiological evidence for caudal brainstem projections of jaw muscle spindle afferents. Exp Brain Res 128:425–434
Suter KJ, Smith BN, Dudek FE (1999) Electrophysiological recording from brain slices. Methods 18:86–90
Wilson MA, McNaughton BL (1993) Dynamics of the hippocampal ensemble code for space. Science 261:1055–1058
Zhang JD, Yoshida A, Shigenaga Y (1997) Ultrastructural analysis of inputs around the soma of an intra-cellularly labeled masseter muscle spindle afferent in cat mesencephalic trigeminal nucleus. J Hirnforsch 38:495–502
Zhang J, Liang H, Luo P, Xiong H (2011) Unraveling a masticatory—oculomotor neural pathway in rat: implications for a pathophysiological neural circuit in human? Int J Physiol Pathophysiol Pharmacol 3:280–287
Zhang J, Luo P, Ro JY, Xiong H (2012) Jaw muscle spindle afferents coordinate multiple orofacial motoneurons via common premotor neurons in rats: an electrophysiological and anatomical study. Brain Res 1489:37–47
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Zhang, J., Xia, J., Xiong, H. (2014). Techniques for Extracellular Recordings. In: Xiong, H., Gendelman, H.E. (eds) Current Laboratory Methods in Neuroscience Research. Springer Protocols Handbooks. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-8794-4_23
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DOI: https://doi.org/10.1007/978-1-4614-8794-4_23
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