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Confocal Imaging of Nerve Cells

  • You ZhouEmail author
Protocol
  • 3.9k Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Confocal laser scanning microscopy has been widely utilized for real-time, cytochemical, or immunofluorescence image analysis in many studies using various types of biological samples. Recently developed fluorophores and fluorescent proteins with higher fluorescence intensities in narrower ranges of excitation and emission wavelengths give researchers more choices for selections of fluorescent tags and labeling combinations. This chapter provides brief introduction of confocal microscopy, autofluorescence with focus on aging brains, signal and noise ratio, and common fluorophore selections/combinations. In addition, this paper describes general procedures for confocal image analysis using live cells or fixed samples, using examples for (1) real-time imaging analysis of intracellular ROS response of Neuro-2A cells to specific treatments and (2) triple-labeling immunofluorescence confocal microscopy using mouse brain sections.

Keywords

Autofluorescence Confocal laser scanning microscopy Fluorophores and fluorescent proteins selection Immunofluorescence labeling Nerve cells Real-time imaging Signal-noise ratio 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Center for Biotechnology and School of Veterinary Medicine & Biomedical SciencesUniversity of Nebraska-LincolnLincolnUSA

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