Polymerase Chain Reaction (PCR) and Real-Time PCR

  • Georgette D. KanmogneEmail author
Part of the Springer Protocols Handbooks book series (SPH)


The PCR technique, developed over 3 decades ago, has revolutionized molecular biology. PCR applications encompass several fields, including genetics, molecular cloning, diagnosis of infectious and hereditary diseases, and forensic sciences. Improvements of the PCR technique have resulted in its expansion to include variants such as reverse-transcription PCR, real-time PCR, and real-time reverse-transcription PCR. This chapter provides an overview of current PCR and PCR variants methods.


PCR Quantitative PCR mRNA Complementary DNA Reverse transcription 



Dr. Georgette Kanmogne’s research is supported by grants from the National Institute of Mental Health grants 1RO1 MH081780 and 1RO1 MH094160.


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© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of Pharmacology and Experimental NeuroscienceUniversity of Nebraska Medical CenterOmahaUSA

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