Polymerase Chain Reaction (PCR) and Real-Time PCR
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The PCR technique, developed over 3 decades ago, has revolutionized molecular biology. PCR applications encompass several fields, including genetics, molecular cloning, diagnosis of infectious and hereditary diseases, and forensic sciences. Improvements of the PCR technique have resulted in its expansion to include variants such as reverse-transcription PCR, real-time PCR, and real-time reverse-transcription PCR. This chapter provides an overview of current PCR and PCR variants methods.
KeywordsPCR Quantitative PCR mRNA Complementary DNA Reverse transcription
Dr. Georgette Kanmogne’s research is supported by grants from the National Institute of Mental Health grants 1RO1 MH081780 and 1RO1 MH094160.
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