Abstract
Flow cytometry has been used for numerous applications in immunology, cell biology, and neuroscience research (de Graaf, et al. 2011). With the development of higher sensitivity instrumentation and smaller numbers of cells required, flow cytometry has become an adjunctive methodology for studies of the interface between peripheral immune cells and the nervous system Schwatrz and Kipnis, 2011 (Ref). In this chapter, we will explore the use of flow cytometry to assess and isolate lymphoid cells associated with neuronal injury as well as how this approach would prove helpful in on the identification of neuronal cells. In addition, isolation of cells by other means will be discussed.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Badie B, Schartner JM, Paul J, Bartley BA, Jl V, Preston K (2000) Dexamethasone-induced abolition of the inflammatory response in an experimental glioma model: a flow cytometry study. J Neurosurg 93:634–639
Behbahani H, Rickle A, Concha H, Ankarcrona M, Winblad B, Cowburn RF (2005) Flow cytometry as a method for studying effects of stressors on primary rat neurons. J Neurosci Res 82:432–441
Bhattacharya S, Jackson JD, Das AV, Thoreson WB, Kuszynski C, James J, Joshi S, Ahmad I (2002) Direct identification and enrichment of retinal stem cells/progenitors by Hoechst 33342 dye efflux assay. Invest Opthalmol Vis Sci 44(6):2764–2773
Bilsland JG, Haldon C, Goddard J, Oliver K, Murray F, Wheeldon A, Cumberbatch J, McAllister G, Munoz-Sanjuan I (2006) A rapid method for the quantification of mouse hippocampal neurogenesis in vivo by flow cytometry: validation with conventional and enhanced immunohistochemical methods. J Neurosci Methods 157:54–63
de Graaf MT, de Jongste AHC, Kraan J, Boonstra JG, Sillevis Smitt PAE, Gratama JW (2011) Flow cytometric characterization of cerebrospinal fluid cells. Cytometry B Clin Cytom 80B:271–281
Gylys KH, Fein JA, Cole GM (2000) Quantitative characterization of crude synaptosomal fraction (P-2) components by flow cytometry. J Neurosci Res 61:186–192
Jamur MC, Oliver C (2010) Permeabilization of cell membranes. Methods Mol Biol 588:63–66
Jung SS, Nalbantoglu J, Cashman NR (1996) Alzheimer’s beta-amyloid precursor protein is expressed on the surface of immediately ex vivo brain cells: a flow cytometric study. J Neurosci Res 46:336–348
Kawaguchi A, Miyata T, Sawamoto K, Takashita N, Murayama A, Akamatsu W, Ogawa M, Okabe M, Tano Y, Goldman S, Okano H (2001) Nestin-EGFP transgenic mice: visualization of the self-renewal and multipotency of CNS stem cells. Mol Cell Neurosci 17:259–273
Kipnis J, Gadani S, Derecki NC (2012 Sep) Pro-cognitive properties of T cells. Nat Rev Immunol 12(9):663–669. doi:10.1038/nri3280. Epub 2012 Aug 20, PubMed PMID: 22903149
Klassen H, Schwartz MR, Bailey A, Young M (2001) Surface markers expressed by multipotent human and mouse neural progenitor cells include tetraspanins and non-protein epitopes. Neurosci Lett 312:180–182
Kraan J, Gratama JW, Haioun C, Orfao A, Plonquet A, Porwit A, Quijano S, Stetler-Stevenson M, Subira D, Wilson W (2008) Flow cytometric immunophenotyping of cerebrospinal fluid. Curr Protoc Cytom Chapter 6:Unit 6.25
McLarena FH, Svendsenb CN, Van der Meidec P, Jolya E (2001) Analysis of neural stem cells by flow cytometry: cellular differentiation modifies patterns of MHC expression. J Neuroimmunol 112:35–46
Mosley RL, Hutter-Saunders JA, Stone DK, Gendelman HE (2012) Inflammation and adaptive immunity in Parkinson's disease. Cold Spring Harb Perspect Med 2(1):a009381, PMID:22315722
Neurauter AA et al (2007) Cell isolation and expansion using Dynabeads®. Adv Biochem Eng Biotechnol 106:41–73
Schwartz M, Kipnis JA (2011) A conceptual revolution in the relationships between the brain and immunity. Brain Behav Immun 25(5):817–819, PubMed PMID: 21187141; PubMed Central PMCID: PMC3074045
Stevens SL, Bao J, Hollis J, Lessov NS, Clark WM, Stenzel-Poorea MP (2002) The use of flow cytometry to evaluate temporal changes in inflammatory cells following focal cerebral ischemia in mice. Brain Res 932:110–119
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2014 Springer Science+Business Media New York
About this protocol
Cite this protocol
Kuszynski, C. (2014). Flow Cytometry in Neuroscience Research. In: Xiong, H., Gendelman, H.E. (eds) Current Laboratory Methods in Neuroscience Research. Springer Protocols Handbooks. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-8794-4_13
Download citation
DOI: https://doi.org/10.1007/978-1-4614-8794-4_13
Published:
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4614-8793-7
Online ISBN: 978-1-4614-8794-4
eBook Packages: Springer Protocols