Flow Cytometry in Neuroscience Research

  • Charles KuszynskiEmail author
Part of the Springer Protocols Handbooks book series (SPH)


Flow cytometry has been used for numerous applications in immunology, cell biology, and neuroscience research (de Graaf, et al. 2011). With the development of higher sensitivity instrumentation and smaller numbers of cells required, flow cytometry has become an adjunctive methodology for studies of the interface between peripheral immune cells and the nervous system Schwatrz and Kipnis, 2011 (Ref). In this chapter, we will explore the use of flow cytometry to assess and isolate lymphoid cells associated with neuronal injury as well as how this approach would prove helpful in on the identification of neuronal cells. In addition, isolation of cells by other means will be discussed.


Flow cytometry neuroscience Immunological staining 


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© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of Pathology and MicrobiologyUniversity of Nebraska Medical CenterOmahaUSA
  2. 2.Cell Analysis FacilityUniversity of Nebraska Medical CenterOmahaUSA

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