Xenopus Oocytes Isolation and Microinjection
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Xenopus oocytes, obtained from sexually mature female Xenopus laevis, are widely used as a model system for expression cloning and structure-function studies of ion channels and receptors. The ovarian tissue can be extracted either by a nonfatal surgical removal of parts of the ovary on an anesthetized frog several times during its lifetime or by removal of the ovary from the frog decapitated after deep anesthesia. Then, the Xenopus oocytes can be isolated and defolliculated manually or enzymatically or combined. Healthy-looking oocytes in stage V and/or VI are selected for microinjection of RNA or DNA. This chapter describes the methodology and procedures of surgical removal of ovarian tissue, enzymatic isolation and defolliculation of Xenopus oocytes, and oocyte microinjection.
KeywordsXenopus laevis Xenopus oocytes Oocyte isolation Defolliculation Oocyte microinjection
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