Abstract
Intrinsically disordered regions (IDRs) of proteins are very thin and hence hard to be visualized by electron microscopy. Thus far, only high-speed atomic force microscopy (HS-AFM) can visualize them. The molecular movies identify the alignment of IDRs and ordered regions in an intrinsically disordered protein (IDP) and show undulation motion of the IDRs. The visualized tail-like structures contain the information of mechanical properties of the IDRs. Here, we describe methods of HS-AFM visualization of IDPs and methods of analyzing the obtained images to characterize IDRs.
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Acknowledgment
This work was supported by Grant-in-Aid for Basic Research (S) from JSPS, Knowledge Cluster/MEXT—Japan, and Grant-in Aid for Scientific Research on Innovative Areas (Research in a Proposed Research Area)/MEXT—Japan.
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Ando, T., Kodera, N. (2012). Visualization of Mobility by Atomic Force Microscopy. In: Uversky, V., Dunker, A. (eds) Intrinsically Disordered Protein Analysis. Methods in Molecular Biology, vol 896. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-3704-8_4
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DOI: https://doi.org/10.1007/978-1-4614-3704-8_4
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