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Primary Isolation of Mycobacterium ulcerans

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Mycobacterium ulcerans

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2387))

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Abstract

Primary isolation of Mycobacterium ulcerans is the separation and growth of the bacterium from a mixed population either in clinical specimen or environmental specimen in pure cultures. It is a crucial activity as it can be used to monitor antimicrobial treatment, surveillance for antimicrobial resistance, and molecular epidemiology studies toward understanding pathogen ecology and transmission as well as pathogen biology. The process involves removal of unwanted fast-growing bacteria using 5% oxalic acid, inoculation on Lowenstein-Jensen medium supplemented with glycerol, and incubation at temperatures between 30 °C and 33 °C.

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Author information

Authors and Affiliations

  1. Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana

    Dorothy Yeboah-Manu & Adwoa Asante-Poku

  2. Institute for Environmental and Sanitation Studies, University of Ghana, Accra, Ghana

    Sammy Yaw Aboagye

Authors
  1. Dorothy Yeboah-Manu
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  2. Adwoa Asante-Poku
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  3. Sammy Yaw Aboagye
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Corresponding author

Correspondence to Dorothy Yeboah-Manu .

Editor information

Editors and Affiliations

  1. Swiss Tropical and Public Health Institute, Basel, Switzerland

    Gerd Pluschke

  2. Stanford School of Medicine Department of Pathology, Stanford University, Stanford, CA, USA

    Katharina Röltgen

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Yeboah-Manu, D., Asante-Poku, A., Yaw Aboagye, S. (2022). Primary Isolation of Mycobacterium ulcerans. In: Pluschke, G., Röltgen, K. (eds) Mycobacterium ulcerans. Methods in Molecular Biology, vol 2387. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1779-3_3

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  • DOI: https://doi.org/10.1007/978-1-0716-1779-3_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1778-6

  • Online ISBN: 978-1-0716-1779-3

  • eBook Packages: Springer Protocols

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