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Virus-Induced Gene Silencing in Plants pp 65–74Cite as

Virus-Induced Gene Silencing of Cell Wall Genes in Flax (Linum usitatissimum)

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2172))

Abstract

Plants have developed defense mechanisms against viruses by using an RNA silencing-based process, which has many common features with the endogenous RNA silencing pathway used for regulating the level of transcripts derived from developmental genes. In the virus-induced gene silencing (VIGS) method, it is possible to take advantage of this mechanism by inserting a plant nucleic fragment within the viral genome to knock down the corresponding gene. This tool has been used in many species as a fast and easy reverse genetics technique in order to gain information on the role of genes with poorly understood functions. Here we describe in detail two Agrobacterium-mediated infection protocols in flax, based on a whole plant vacuum infiltration and a leaf syringe infiltration that systemically impact the transcript levels in the stem.

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Author information

Authors and Affiliations

  1. Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, Umeå, Sweden

    Maxime Chantreau

  2. UMR CNRS 8576—UGSF, Université de Lille, Villeneuve d’Ascq, France

    Godfrey Neutelings

Authors
  1. Maxime Chantreau
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  2. Godfrey Neutelings
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Corresponding authors

Correspondence to Maxime Chantreau or Godfrey Neutelings .

Editor information

Editors and Affiliations

  1. EA2106 Biomolécules et Biotechnologies Végétales, Université de Tours, Tours, France

    Vincent Courdavault

  2. EA2106 Biomolécules et Biotechnologies Végétales, Université de Tours, Tours, France

    Sébastien Besseau

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Chantreau, M., Neutelings, G. (2020). Virus-Induced Gene Silencing of Cell Wall Genes in Flax (Linum usitatissimum). In: Courdavault, V., Besseau, S. (eds) Virus-Induced Gene Silencing in Plants. Methods in Molecular Biology, vol 2172. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0751-0_6

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  • DOI: https://doi.org/10.1007/978-1-0716-0751-0_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0750-3

  • Online ISBN: 978-1-0716-0751-0

  • eBook Packages: Springer Protocols

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