Abstract
Fluorescence microscopy is currently one of the more powerful and versatile techniques available for biological studies. With conventional biological immunofluorescence microscopy, caveolin-1 (CAV1) is visualized as numerous small dots, which are often distributed as a linear array or along the edge of the cell. Although its presence, as well as that of other proteins, can be detected by conventional immunofluorescence microscopy, those results do not clarify whether two different proteins exist in the plasma membrane of a specimen or how they are distributed two-dimensionally. Here, we describe an unroofing procedure that clearly reveals CAV1 localization in a single plane of the plasma membrane and also demonstrate a super-resolution structured illumination microscopy technique for observation of CAV1 in the plasma membrane.
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Acknowledgments
This work was supported in part by Grants-in-Aid for Scientific Research (A) and (C) from the Japan Society for the Promotion of Science (JSPS), and Project for Cancer Research and Therapeutic Evolution (P-CREATE) program of the Japan Agency for Medical Research and Development (AMED), as well as a Grant-in-Aid for Scientific Research on Innovative Areas from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan.
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Yamaguchi, T., Fujimoto, T., Takahashi, T. (2020). Method for Efficient Observation of Caveolin-1 in Plasma Membrane by Microscopy Imaging Analysis. In: Blouin, C. (eds) Caveolae. Methods in Molecular Biology, vol 2169. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0732-9_4
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DOI: https://doi.org/10.1007/978-1-0716-0732-9_4
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