Abstract
Caveolae are small flask- or cup-shaped invaginations of the plasma membrane found in almost all vertebrate cells. Due to their small size (50–100 nm), transmission electron microscopy (TEM) has been the method of choice to study caveolae formation and ultrastructure and, more recently, to resolve the sub-caveolar localization of its protein components using novel protein labeling methods for TEM. This chapter describes a protocol for the selective visualization of caveolae and caveolar proteins by TEM, 3D tomography, and correlative light and electron microscopy (CLEM) using the peroxidase APEX2.
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Acknowledgments
This work was supported by grants from MOE Singapore (TIER1 RG39-14) and Nanyang Technological University (NIM/03/2016).
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Ludwig, A. (2020). Selective Visualization of Caveolae by TEM Using APEX2. In: Blouin, C. (eds) Caveolae. Methods in Molecular Biology, vol 2169. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0732-9_1
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DOI: https://doi.org/10.1007/978-1-0716-0732-9_1
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Publisher Name: Humana, New York, NY
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