Engraftment of Human Hepatocytes in the PiZ-NSG Mouse Model

Part of the Methods in Molecular Biology book series (MIMB, volume 2164)


We recently described the generation of a novel mouse strain that efficiently and readily engrafts human primary hepatocytes to create liver xenografts (Borel et al., Mol Ther, 25: 2477–89, 2017). A transgenic mouse strain expressing a human PiZ allele for the SerpinA1 gene was crossed with the NOD-SCID-gamma chain knockout (NSG) strain to create a recipient strain (PiZ-NSG) for human hepatocyte xenotransplantation. In this chapter we provide a description of the methods to achieve these liver xenografts in the PiZ-NSG mouse.

Key words

Hepatectomy Human hepatocyte Engraftment Intrasplenic injection Humanized mouse model Liver xenograft Liver regeneration Hepatocyte isolation 



The mouse liver diagram in Fig. 1 was created by Mr. Hong Ma, Gene Therapy Center University of Massachusetts Medical School.


  1. 1.
    Borel F, Tang Q, Gernoux G, Greer C, Wang Z, Barzel A et al (2017) Survival advantage of both human hepatocyte xenografts and genome-edited hepatocytes for treatment of alpha-1 antitrypsin deficiency. Mol Ther 25(11):2477–2489CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Gene Therapy Center, University of Massachusetts Medical SchoolWorcesterUSA
  2. 2.Department of PediatricsGene Therapy Center, University of Massachusetts Medical SchoolWorcesterUSA
  3. 3.West China School of MedicineSichuan UniversityChengduChina
  4. 4.Microbiology and Physiologic SystemsGene Therapy Center, University of Massachusetts Medical SchoolWorcesterUSA

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