Engraftment of Human Hepatocytes in the PiZ-NSG Mouse Model
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We recently described the generation of a novel mouse strain that efficiently and readily engrafts human primary hepatocytes to create liver xenografts (Borel et al., Mol Ther, 25: 2477–89, 2017). A transgenic mouse strain expressing a human PiZ allele for the SerpinA1 gene was crossed with the NOD-SCID-gamma chain knockout (NSG) strain to create a recipient strain (PiZ-NSG) for human hepatocyte xenotransplantation. In this chapter we provide a description of the methods to achieve these liver xenografts in the PiZ-NSG mouse.
Key wordsHepatectomy Human hepatocyte Engraftment Intrasplenic injection Humanized mouse model Liver xenograft Liver regeneration Hepatocyte isolation
The mouse liver diagram in Fig. 1 was created by Mr. Hong Ma, Gene Therapy Center University of Massachusetts Medical School.