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Kupffer Cell Protein Isolation and Detection by Western Blot

Protocol
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Part of the Methods in Molecular Biology book series (MIMB, volume 2164)

Abstract

Kupffer cells are the majority of tissue macrophages present in the body and are actively involved in the regulation of liver homeostasis as well as in the progression of liver diseases. Here we describe a methodology to successfully isolate and detect proteins from Kupffer cells and avoid the contamination of other liver cell populations.

Key words

Liver Kupffer cells Proteins Protein isolation Western blot SDS-PAGE 

References

  1. 1.
    Duarte N, Coelho IC, Patarrão RS et al (2015) How inflammation impinges on NAFLD: a role for Kupffer cells. Biomed Res Int 2015:984578CrossRefGoogle Scholar
  2. 2.
    Kawai T, Akira S (2009) The roles of TLRs, RLRs and NLRs in pathogen recognition. Int Immunol 21:317–337CrossRefGoogle Scholar
  3. 3.
    Ramachandran P, Pellicoro A, Vernon MA et al (2012) Differential Ly-6C expression identifies the recruited macrophage phenotype, which orchestrates the regression of murine liver fibrosis. Proc Natl Acad Sci U S A 109:E3186–E3195CrossRefGoogle Scholar
  4. 4.
    Tacke F, Zimmermann HW et al (2014) Macrophage heterogeneity in liver injury and fibrosis. J Hepatol 60(5):1090–1096CrossRefGoogle Scholar
  5. 5.
    Zimmermann HW, Trautwein C, Tacke F et al (2012) Functional role of monocytes and macrophages for the inflammatory response in acute liver injury. Front Physiol 3:56CrossRefGoogle Scholar
  6. 6.
    Morgantini C, Jager J, Li X et al (2019) Liver macrophages regulate metabolism through non-inflammatory factors. Nat Metab 1:445–459CrossRefGoogle Scholar
  7. 7.
    Stienstra R, Saudale F, Duval C, Keshtkar S et al (2010) Kupffer cells promote hepatic steatosis via interleukin-1beta-dependent suppression of peroxisome proliferator-activated receptor alpha activity. Hepatology 51(2):511–522CrossRefGoogle Scholar
  8. 8.
    Tencerova M, Aouadi M, Vangala P et al (2015) Activated Kupffer cells inhibit insulin sensitivity in obese mice. FASEB J 29(7):2959–2969.  https://doi.org/10.1096/fj.15-270496CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Aparicio-Vergara M, Tencerova M, Morgantini C, Barreby E, Aouadi M (2017) Isolation of Kupffer cells and hepatocytes from a single mouse liver. Methods Mol Biol 1639:161–171CrossRefGoogle Scholar
  10. 10.
    Borel F, Mueller C (eds) (1639) Alpha-1 antitrypsin deficiency. Methods in molecular biology. Humana Press, New YorkGoogle Scholar
  11. 11.
    Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Medicine, Integrated Cardio Metabolic Center, Karolinska InstitutetHuddingeSweden

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