Kupffer Cell Protein Isolation and Detection by Western Blot

Part of the Methods in Molecular Biology book series (MIMB, volume 2164)


Kupffer cells are the majority of tissue macrophages present in the body and are actively involved in the regulation of liver homeostasis as well as in the progression of liver diseases. Here we describe a methodology to successfully isolate and detect proteins from Kupffer cells and avoid the contamination of other liver cell populations.

Key words

Liver Kupffer cells Proteins Protein isolation Western blot SDS-PAGE 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Medicine, Integrated Cardio Metabolic Center, Karolinska InstitutetHuddingeSweden

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