Abstract
Kupffer cells and hepatocytes maintain liver homeostasis. These cells could be separated based on their size and weight, by centrifugation using a density gradient after a liver perfusion. Here, we describe a methodology to isolate both Kupffer cells and hepatocytes from a single mouse, which provides the unique advantage of studying these two cell types from the same liver.
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Oses, C., Aouadi, M. (2020). Kupffer Cell and Hepatocyte Isolation from a Single Mouse Liver by Gradient Centrifugation. In: Aouadi, M., Azzimato, V. (eds) Kupffer Cells. Methods in Molecular Biology, vol 2164. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0704-6_1
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DOI: https://doi.org/10.1007/978-1-0716-0704-6_1
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-0704-6
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