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Kupffer Cell and Hepatocyte Isolation from a Single Mouse Liver by Gradient Centrifugation

Protocol
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Part of the Methods in Molecular Biology book series (MIMB, volume 2164)

Abstract

Kupffer cells and hepatocytes maintain liver homeostasis. These cells could be separated based on their size and weight, by centrifugation using a density gradient after a liver perfusion. Here, we describe a methodology to isolate both Kupffer cells and hepatocytes from a single mouse, which provides the unique advantage of studying these two cell types from the same liver.

Key words

Liver Kupffer cells Hepatocytes Cell isolation Perfusion Gradient centrifugation 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Medicine, Integrated Cardio Metabolic CenterKarolinska InstitutetHuddingeSweden

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