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Kupffer Cells pp 121-127 | Cite as

An Obesogenic Dietary Mouse Model of Nonalcoholic Steatohepatitis

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Part of the Methods in Molecular Biology book series (MIMB, volume 2164)

Abstract

Nonalcoholic steatohepatitis (NASH) is a severe form of nonalcoholic fatty liver disease (NAFLD), characterized by steatosis (fat within the liver), inflammation, and fibrosis, which may progress to cirrhosis and hepatocellular carcinoma. Despite the high prevalence, there are currently no approved NASH drug treatments, which urges a faster development of new therapies to address this high unmet medical need. Drug development is facilitated by having reliable and translatable preclinical NASH models. Obesogenic dietary models recapitulate better the natural progression of NASH, with overnutrition and sedentary lifestyle being the main causes. Here we describe the use of a modified version of a diet-induced NASH model, known as the Amylin NASH diet model (AMLN-diet), particularly in the leptin-deficient Lepob/Lepob (ob/ob) mice.

Key words

NAFLD-NASH Mouse model Steatosis Inflammation Fibrosis Ballooning-NAS 

Notes

Disclosure

The authors are AstraZeneca employees and declare no competing interests related to this work.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Bioscience Metabolism, Research and Early Development, Cardiovascular, Renal and Metabolic (CVRM), BioPharmaceuticals R&D, AstraZenecaGothenburgSweden
  2. 2.The Lundberg Laboratory for Diabetes ResearchUniversity of GothenburgGothenburgSweden
  3. 3.Wallenberg Centre for Molecular and Translational MedicineUniversity of GothenburgGothenburgSweden

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